DEHYDRATION/REHYDRATION OF DERIVATIZED, MARKER-LOADED LIPOSOMES ON A TEST DEVICE AND METHOD OF USE THEREOF
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Abstract
The present invention relates to a method for making a test device for detecting or quantifying an analyte in a sample. This method involves contacting a membrane with a mixture including derivatized, marker-loaded liposomes, and substantially dehydrating the mixture on the membrane under vacuum pressure at a temperature of from about 4° C. to about 80° C., wherein said mixture further includes one or more sugars in an amount sufficient to promote the stability of the liposomes during dehydration and rehydration. The present invention also relates to a test device and method for detecting or quantifying an analyte in a sample. The test device includes a membrane which includes an immobilized liposome zone, wherein the immobilized liposome zone has bound thereto dehydrated, derivatized, marker-loaded liposomes dehydrated under vacuum pressure at a temperature of from about 4° C. to about 80° C. from a mixture which includes one or more sugars in an amount sufficient to promote the stability of the liposomes during dehydration and rehydration.
99 Citations
57 Claims
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1-41. -41. (canceled)
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42. A test device for detecting or quantifying an analyte in a test sample, the test device comprising a membrane, the membrane comprising:
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a contact portion at or proximate to a first end of the membrane; an electrochemical measurement portion at a location on the membrane which is positioned away from the first end, wherein the electrochemical measurement portion comprises an electrochemical detector cell comprising a first conductor having a plurality of fingers, and a second conductor having a plurality of fingers, wherein the fingers of the first conductor are interdigitated with the fingers of the second conductor and are positioned on a glass substrate, and wherein the conductors induce redox cycling; and a liposome lysing portion segregated from the contact portion and having a liposome lysing agent bound thereto, wherein the liposome lysing portion either is positioned between the contact portion and the electrochemical measurement portion, or partially or completely coincides with the electrochemical measurement portion. - View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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57-60. -60. (canceled)
Specification