Microfluidic Devices and Methods of Using Same
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Abstract
A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
125 Citations
25 Claims
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1-13. -13. (canceled)
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14. A method of analyzing a sample fluid for a reaction comprising the steps of:
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(i) providing a microfluidic device, said microfluidic device comprising; a plurality of fluidic channels, each said channel having more than two valves associated therewith, which valves, when actuated, partition said channel into more than two reaction sites, wherein each said reaction site is delimited by exactly two actuated valves; an inlet in communication with said channel, said inlet for introducing said sample fluid; and
,wherein when said sample fluid is introduced into said fluidic channel, and said valves are actuated, said sample fluid is divided into separate sample fluid portions; (ii) introducing said sample fluid into said inlet, said sample fluid filling said channel; (iii) actuating said valves to separate said sample fluid into more than two separate portions within said channel; (iv) simultaneously thermocycling said separate portions of sample fluid; (v) analyzing said separate portions of sample fluid to determine whether said thermocycling produced said reaction. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification