Methods and Apparatus for Creating Particle Derivatives of HDL with Reduced Lipid Content

• US 20080230465A1
• Filed: 03/24/2008
• Published: 09/25/2008
• Est. Priority Date: 07/03/2003
• Status: Abandoned Application

• Current Assignee: Lipid Sciences, Inc.
• Original Assignee: Lipid Sciences, Inc.
• Inventors: Perlman, Timothy Jon, Conner, Adam Paul, Bellotti, Marc, Akeefe, Hassibullah, Brewer, H. Bryan
• Application Number:
  US12/054,088
• Publication Number:
  US 20080230465A1
• Time in Patent Office: Days
• US Class Current: 210/251
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/1709 from mammals
• CPC Class Codes: A61K 9/1275 Lipoproteins; Chylomicrons; Artificial HDL, LDL, VLDL, protein-free species thereof; Precursors thereof
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/04 Anorexiants; Antiobesity agents
• CPC Class Codes: A61P 3/06 Antihyperlipidemics
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: A61P 9/12 Antihypertensives
• CPC Class Codes: A61P 9/14 Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
• CPC Class Codes: C07K 14/775 Apolipopeptides
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743

Abstract

The present invention is directed to systems, apparatus and methods for creating derivatives of at least one form of HDL without substantially affecting LDL. These derivatives of HDL are particles with reduced lipid content, particularly reduced cholesterol content. These particles have the capacity to bind cholesterol and are administered to a patient to enhance cellular cholesterol efflux and reduce cholesterol levels in cells, tissues, organs, and blood vessels. The present method is useful for treating atherogenic vascular disease and may be combined with other therapies such as statins, inhibitors of cholesterol absorption, niacin, anti-inflammatories, exercise and dietary restriction.

Claims

1-62. -62. (canceled)

63. A kit comprising:

a. a high density lipoprotein source container;

b. a lipid removing agent source container;

c. a mixer comprising at least one of a static mixer, vortexer, or centrifuge;

d. a separator comprising at least one of an absorbent, separator, centrifuge, or charcoal column;

e. an output container for storing a particle derivative of the high density lipoprotein; and

,

f. a plurality of tubing and a plurality of valves for controlling the flow of high density lipoprotein from the high density lipoprotein source container and lipid removing agent from the lipid removing agent source container to the mixer, for controlling the flow of mixture of lipid removing agent, lipid, and particle derivative of the high density lipoprotein to the separator, and for controlling the flow of the particle derivative of the high density lipoprotein to the output container.

64. A kit comprising:

a. a high density lipoprotein source container;

b. a lipid removing agent source container;

c. a mixer comprising at least one of a static mixer, vortexer, or centrifuge;

d. a separator comprising at least one of an absorbent, separator, centrifuge, or charcoal column;

e. an output container for storing modified high density lipoprotein; and

,

f. a plurality of tubing and a plurality of valves for controlling the flow of high density lipoprotein from the high density lipoprotein source container and lipid removing agent from the lipid removing agent source container to the mixer, for controlling the flow of mixture of lipid removing agent, lipid, and modified high density lipoprotein to the separator, and for controlling the flow of modified high density lipoprotein to the output container.

65-72. -72. (canceled)