RNAi MODULATION OF MLL-AF4 AND USES THEREOF
First Claim
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1. A method of reducing the amount of MLL-AF4 RNA in a cell or of inhibiting the rate of proliferation of t(4;
- 11)-positive cells, comprising the step of;
contacting the cell or cells with an iRNA agent.
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Abstract
The invention relates to compositions and methods for modulating the expression of the MLL-AF4 fusion gene, and more particularly to the downregulation of MLL-AF4 by chemically modified oligonucleotides.
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13 Claims
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1. A method of reducing the amount of MLL-AF4 RNA in a cell or of inhibiting the rate of proliferation of t(4;
- 11)-positive cells, comprising the step of;
contacting the cell or cells with an iRNA agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13)
- 11)-positive cells, comprising the step of;
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8. A method of evaluating an iRNA agent thought to inhibit expression of an MLL-AF4 gene, the method comprising:
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(a) providing an iRNA agent, wherein a first strand of the iRNA agent is sufficiently complementary to a nucleotide sequence of an MLL-AF4 mRNA, and a second strand of the iRNA agent is sufficiently complementary to the first strand to hybridize to the first strand; (b) contacting the iRNA agent to a cell comprising an MLL-AF4 gene; (c) comparing MLL-AF4 gene expression before contacting the iRNA agent to the cell, or of uncontacted control cells, to MLL-AF4 gene expression after contacting the iRNA agent to the cell; and (d) determining whether the iRNA agent is useful for inhibiting MLL-AF4 gene expression, wherein the iRNA is useful if the amount of MLL-AF4 RNA present in the cell, or protein secreted by the cell, is less than the amount present or secreted prior to contacting the iRNA agent to the cell, or less than the amount present or secreted by cells not so contacted.
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Specification