Process for Labeling and Purification of Nucleic Acids of Interest Present in a Biological Sample to be Treated in a Single Reaction Vessel
First Claim
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1. A process for labeling and purification of nucleic acids of interest which are present in a biological sample to be treated, consisting in:
- taking a single reaction vessel,introducing into the reaction vessel;
the biological sample,at least one labeling reagent for nucleic acids, said labeling reagent being stable to heat and of formula (0);
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Abstract
The present invention relates to a process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising:
- taking a single reaction vessel,
- introducing into the reaction vessel:
- the biological sample,
- at least one labeling reagent for nucleic acids,
- at least one solid support enabling the adsorption of said nucleic acids,
- any ingredient necessary for the labeling of the nucleic acids and/or for the immobilization of said nucleic acids on the support,
- incubating the contents of the reaction vessel, and
- isolating the nucleic acids thus labeled.
The invention finds a preferred application in the diagnostics field.
21 Citations
60 Claims
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1. A process for labeling and purification of nucleic acids of interest which are present in a biological sample to be treated, consisting in:
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taking a single reaction vessel, introducing into the reaction vessel; the biological sample, at least one labeling reagent for nucleic acids, said labeling reagent being stable to heat and of formula (0); - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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21. A process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising:
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taking a single reaction vessel, introducing into the reaction vessel; the biological sample, at least one labeling reagent for nucleic acids, the labeling reagent being contacted with the nucleic acids in homogenous solution, in an essentially aqueous buffer, said labeling reagent being stable to heat, and of formula (6); - View Dependent Claims (60)
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22. A process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising:
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taking a single reaction vessel, introducing into the reaction vessel; the biological sample, at least one labeling reagent for nucleic acids, the labeling reagent being contacted with the nucleic acids in homogenous solution, in an essentially aqueous buffer, said labeling reagent being stable to heat, and of formula (25);
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28. A process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising:
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taking a single reaction vessel, introducing into the reaction vessel; the biological sample, at least one labeling reagent for nucleic acids, the labeling reagent enabling the labeling and the fragmentation a single or double-strand nucleic acid according to the following steps; fragmenting the nucleic acid, attaching a label onto at least one of the fragments by means of a labeling reagent selected from the compounds of formula (19); - View Dependent Claims (29, 30, 31, 32, 33, 34)
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35. A process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising:
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taking a single reaction vessel, introducing into the reaction vessel; the biological sample, at least one labeling reagent for nucleic acids, the labeling reagent being contacted in homogeneous solution, in an essentially aqueous buffer, said labeling reagent being stable to heat and of formula (8); - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 45)
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43. A process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising:
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taking a single reaction vessel, introducing into the reaction vessel; the biological sample, at least one labeling reagent for nucleic acids, the heat-stable labeling reagent is contacted in homogenous solution, in an aqueous buffer, and is of formula (13); - View Dependent Claims (44)
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Specification