PANCREATIC STEM CELLS

• US 20080233649A1
• Filed: 03/20/2008
• Published: 09/25/2008
• Est. Priority Date: 03/05/2004
• Status: Abandoned Application

• Current Assignee: Derek Van Der Kooy, Raewyn Seaberg, Simon Smukler
• Original Assignee: Derek Van Der Kooy, Raewyn Seaberg, Simon Smukler
• Inventors: Seaberg, Raewyn, Smukler, Simon, Van der Kooy, Derek
• Application Number:
  US12/052,505
• Publication Number:
  US 20080233649A1
• Time in Patent Office: Days
• US Class Current: 435/378
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 1/18 for pancreatic disorders, e.g. pancreatic enzymes
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 5/48 of the pancreatic hormones
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/415 Wnt; Frizzeled
• CPC Class Codes: C12N 2501/42 Notch; Delta; Jagged; Serrate
• CPC Class Codes: C12N 2503/02 Drug screening
• CPC Class Codes: C12N 2506/22 from pancreatic cells
• CPC Class Codes: C12N 5/0618 Cells of the nervous system
• CPC Class Codes: C12N 5/0619 Neurons
• CPC Class Codes: C12N 5/0622 Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
• CPC Class Codes: C12N 5/0678 Stem cells; Progenitor cells; Precursor cells

Abstract

Pancreatic progenitor cells isolated from the pancreas of a mammal. The invention also includes pancreatic cells or neural cells differentiated from the pancreatic progenitor cells.

Claims

1. A composition comprising isolated clonal pancreatic stem cells from the pancreas of a mammal and serum-free media.

2. Clonal pancreatic stem cells, pancreatic cells and/or neural cells produced from the composition of claim 1.

3. The composition of claim 1, wherein the cells proliferate in the presence of growth factors, Wnt signaling activators or Notch signaling activators.

4. The composition of claim 3, wherein the growth factors comprise EGF and FGF2.

5. The composition of claim 3, wherein the Wnt signaling activator comprises BIO.

7. The composition of claim 1, wherein the clonal pancreatic stem cells express cell markers Pdx-1 and nestin.

8. The composition of claim 7, wherein the clonal pancreatic stem cells further express at least one of the cell markers:

Sox2, Sox3, Mash1, and Ngn3.

9. The composition of claim 1 wherein the cells do not express Pax4.

10. The composition of claim 2 wherein the cells produced from the composition of claim 1 express Nkx2.2.

6. A method for producing isolated clonal stem cell populations from a pancreatic tissue of a mammal, comprising:

dissociating all or part of the tissue into single cells,

culturing the cells in serum-free media for a time period sufficient that each proliferative pancreatic stem cell has repeatedly divided to produce a corresponding clonal cell population,

isolating one of the corresponding clonal cell populations.

11. The method of claim 6, further comprising clonally passaging one of the corresponding clonal cell populations.