TP53 gene expression and uses thereof
First Claim
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1. A method for identifying a gene as an independent prognostic factor specific for a disease, comprising:
- isolating plasma cells from individuals within a population;
extracting nucleic acid from said plasma cells;
hybridizing said nucleic acid to a DNA array to determine expression levels of genes in the plasma cells; and
performing multivariate regression analyses on data obtained from said hybridization, wherein said analysis identifies the gene as an independent prognostic factor specific for a disease.
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Abstract
The present invention is drawn to diagnosis, prognosis and treatment of multiple myeloma. In this regard, the present invention discloses importance of down-regulation of TP3 gene in multiple myeloma and its use as an independent progostic indicator of multiple myeloma. Additionally, the present invention also discloses novel-TP53 associated genes and demonstrates the clinical relevance of these alterations to disease progression.
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Citations
24 Claims
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1. A method for identifying a gene as an independent prognostic factor specific for a disease, comprising:
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isolating plasma cells from individuals within a population; extracting nucleic acid from said plasma cells; hybridizing said nucleic acid to a DNA array to determine expression levels of genes in the plasma cells; and performing multivariate regression analyses on data obtained from said hybridization, wherein said analysis identifies the gene as an independent prognostic factor specific for a disease. - View Dependent Claims (2, 3, 4, 5, 10, 11, 12, 13, 14, 19, 20, 21)
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6. The method of claim 6, wherein the cancer is multiple myeloma.
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7. A method for identifying a gene relevant in prognosis of a disease, comprising:
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isolating plasma cells from individuals within a population; extracting nucleic acid from said plasma cells; hybridizing said nucleic acid to a DNA microarray; and performing log rank test on the data obtained from said hybridization to identify genes that are up-regulated and down-regulated in the plasma, thereby identifying the gene important for prognosis of the disease. - View Dependent Claims (8, 9, 15, 16, 17)
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18. A method for determining prognosis of an individual with multiple myeloma, comprising:
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obtaining plasma cells from the individual; and determining expression of TP53 alone or in combination with one or more genes selected from the group consisting of TRIM13, NADSYN1, TRIM22, AGRN, CENTD2, SESN1, TM7SF2, NICKAP1, COPG, STAT3, ALOX5, APP, ABCB9, GAA, CEP55, BRCA1, ANLN, PYGL, CCNE2, ASPM, SUV39H2, CDC25A, IFIT5, ANKRA2, PHLDB1, TUBA1A, CDCA7, CDCA2, HFE, RIF1, NEIL3, SLC4A7, FXYD5, MCC, MKNK2, KLHL24, DLC1, OPN3, B3GALNT1, SPRED1, ARHGAP25, RTN2, WNT16, DEPDC1, STT3B, ECHDC2, ENPP4, SAT2, SLAMF7, MAN1C1, INTS7, ZNF600, L3MBTL4, LAPTM4B, OSBPL10, KCNS3, THEX1. CYB5D2, UNC93B1, SIDT1, TMEM57, HIGD24, FKSG44, C14orf28, LOC387763, TncRNA, C18orf1, DCUN1D4, FANCI, ZMAT3, NOTCH1, BTG2, RAB1A, TNFRSF10B, HDLBP, RIT1, KIF2C, S100A4, MEIS1, SGOL2, CD302, C5orf34, FAM111B and C18orf54; and comparing the expression level of the gene(s) with expression level of the gene in a control individual such that genes that are up-regulated, down-regulated or a combination thereof compared to gene expression levels in plasma cell of a control individual indicates prognosis of said individual. - View Dependent Claims (22, 23, 24)
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Specification