Sequencing single molecules using surface-enhanced Raman scattering
First Claim
1. An apparatus, comprising:
- a fluidic channel configured to receive one or more polymeric biomolecules;
one or more preconfigured resonant pole nano-structures disposed therein said fluidic channel;
means optically coupled with said preconfigured resonant pole nano-structures and adjacent said one or more desired polymeric biomolecules for identifying a Raman induced spectra.
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Abstract
A surface-enhanced Raman scattering method and apparatus to sequence polymeric biomolecules such as DNA, RNA, or proteins is introduced. The method uses metallic nanostructures such as, for example, spherical or cylindrical Au or Ag nanoparticles having characteristic lengths of 10-100 nm which when illuminated with light of the appropriate wavelength produce resonant oscillations of the conduction electrons (plasmon resonance). Electric field enhancements of 30-1000 near the particle surface resulting from such oscillations increase Raman scattering cross-sections by about 106-1015 due to the E4 dependence of the Raman scattering, wherein the largest enhancements occur in the gap/junction between novel closely spaced structures as disclosed herein.
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Citations
58 Claims
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1. An apparatus, comprising:
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a fluidic channel configured to receive one or more polymeric biomolecules; one or more preconfigured resonant pole nano-structures disposed therein said fluidic channel; means optically coupled with said preconfigured resonant pole nano-structures and adjacent said one or more desired polymeric biomolecules for identifying a Raman induced spectra. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 22)
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- 18. The apparatus of 1, wherein both ends of said one or more polymeric biomolecules comprises a dielectric coupled bead, wherein said dielectric coupled beads are manipulated by a dual-optical trap so that said one or more polymeric biomolecules can be immobilized and stretched.
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19. The apparatus of 1, wherein both ends of said one or more polymeric biomolecules comprises a magnetic coupled bead, wherein said magnetic coupled beads are manipulated by a magnetic field so that said one or more polymeric biomolecules can be immobilized and stretched.
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23. An apparatus, comprising:
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a fluidic channel configured to receive one or more polymeric biomolecules; one or more preconfigured wedged resonant nano-structures disposed therein said fluidic channel; and means optically coupled with said wedged resonant structure and adjacent said one or more desired polymeric biomolecules for identifying a Raman induced spectra. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 39)
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33. A sequencing method, comprising:
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directing one or more polymeric biomolecules therethrough a fluidic channel; sequentially probing the nucleotides along said one or more polymeric biomolecules by way of preconfigured resonant pole nano-structures; and optically identifying said probed nucleotides by way of Raman induced spectra. - View Dependent Claims (34, 35, 36, 37, 38, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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50. A sequencing method, comprising:
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directing one or more polymeric biomolecules therethrough a fluidic channel; sequentially probing the nucleotides along said one or more polymeric biomolecules by way of one or more preconfigured wedged nano-structures; and optically identifying said probed nucleotides by way of Raman induced spectra. - View Dependent Claims (51, 52, 53, 54, 55, 56, 57, 58)
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Specification