Nucleic Acid Assays

• US 20080240985A1
• Filed: 07/19/2007
• Published: 10/02/2008
• Est. Priority Date: 05/02/1997
• Status: Abandoned Application

• Current Assignee: Biomerieux Incorporated (Compagnie Merieux Alliance SAS)
• Original Assignee: Biomerieux Incorporated (Compagnie Merieux Alliance SAS)
• Inventors: Catanzariti, Luigi, Kluttz, Bryan W., McKinley, Geoff A., Moe, James G., Burg, J. Lawrence, Vera-Garcia, Marcela
• Application Number:
  US11/780,164
• Publication Number:
  US 20080240985A1
• Time in Patent Office: Days
• US Class Current: 422/68.1
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6846 Common amplification features
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer
• CPC Class Codes: C12Q 2537/125 Sandwich assay format
• CPC Class Codes: C12Q 2545/101 with an internal standard/control
• CPC Class Codes: C12Q 2565/519 characterised by the capture moiety being a single stranded oligonucleotide
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: C12Q 2600/166 Oligonucleotides used as internal standards, controls or normalisation probes
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.

Claims

1. A device for the automated detection of a first target nucleic acid and a second target nucleic acid, said apparatus comprising a solid phase receptacle, wherein said receptacle is coated with a first capture nucleic acid which can form a specific hybridization complex with said first target nucleic acid, and a second capture nucleic acid which can form a specific hybridization complex with said second target nucleic acid.

2. The device of claim 1 wherein said first capture nucleic acid and said second capture nucleic acid are coated on said solid phase receptacle in two distinct zones.

3. The device of claim 1 wherein first capture nucleic acid and said second capture nucleic acid are coated on said solid phase receptacle in a single zone.

4. The device of claim 1, modified for the detection of more than two nucleic acids, wherein the device further comprises additional capture nucleic acids which can each form a specific hybridization complex with additional target nucleic acid other than said first or second target nucleic acid in said sample.