Systems for pathogen detection

• US 20080241000A1
• Filed: 03/27/2007
• Published: 10/02/2008
• Est. Priority Date: 03/27/2007
• Status: Abandoned Application

• Current Assignee: Gearbox LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC (Intellectual Ventures LLC)
• Inventors: Lord, Robert W., Jung, Edward K.Y., Levien, Royce A., Malamud, Mark A., Leuthardt, Eric C., Rinaldo, John D., Wood, Lowell L.
• Application Number:
  US11/729,274
• Publication Number:
  US 20080241000A1
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The present disclosure relates to methods, systems, devices, and microfluidic chips that may be used for the detection of pathogens.

Claims

1. A system comprising:

one or more microfluidic chips configured to facilitate detection of one or more pathogen indicators associated with one or more samples; and

one or more detection units configured to detect the one or more pathogen indicators.

5. The system of claim 1, wherein the one or more microfluidic chips configured to facilitate detection of one or more pathogen indicators associated with one or more samples comprise:

one or more microfluidic chips configured to facilitate detection of the one or more pathogen indicators associated with one or more airborne pathogens.

6. The system of claim 1, wherein the one or more microfluidic chips configured to facilitate detection of one or more pathogen indicators associated with one or more samples comprise:

one or more microfluidic chips configured to facilitate detection of the one or more pathogen indicators associated with one or more food products.

7. The system of claim 1, wherein the one or more microfluidic chips configured to facilitate detection of one or more pathogen indicators associated with one or more samples comprise:

one or more microfluidic chips configured to facilitate detection of the one or more pathogen indicators associated with one or more biological products.

10. The system of claim 1, wherein the one or more detection units configured to detect the one or more pathogen indicators comprise:

one or more detection units configured to detect the one or more pathogen indicators that are associated with one or more pathogens that are airborne.

11. The system of claim 1, wherein the one or more detection units configured to detect the one or more pathogen indicators comprise:

one or more detection units configured to detect the one or more pathogen indicators that are associated with one or more food products.

15. The system of claim 1, further comprising:

one or more display units operably associated with the one or more detection units.

18. The system of claim 15, wherein the one or more display units operably associated with the one or more detection units comprise:

one or more display units that indicate a presence or an absence of one or more pathogens within the one or more samples.

19. The system of claim 15, wherein the one or more display units operably associated with the one or more detection units comprise:

one or more display units that indicate an identity of one or more pathogens present within the one or more samples.

20. The system of claim 15, wherein the one or more display units operably associated with the one or more detection units comprise:

one or more display units that indicate one or more concentrations of one or more pathogens within the one or more samples.

21. The system of claim 15, further comprising:

one or more reagent delivery units configured to deliver one or more reagents to the one or more microfluidic chips.

27. The system of claim 21, further comprising:

one or more centrifugation units.

28. The system of claim 27, wherein the one or more centrifugation units comprise:

one or more centrifugation units configured to centrifuge the one or more microfluidic chips that are operably associated with the one or more centrifugation units.

30. The system of claim 27, wherein the one or more centrifugation units comprise:

one or more centrifugation units configured for polynucleotide extraction from the one or more samples.

32. The system of claim 27, further comprising:

one or more reservoir units.

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35. A system comprising:

one or more microfluidic chips that are configured to allow one or more magnetically active pathogen indicator binding agents to bind to one or more pathogen indicators associated with one or more samples to form one or more magnetically active pathogen indicator complexes and separate the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples.

36. The system of claim 35, wherein the one or more microfluidic chips that are configured to allow one or more magnetically active pathogen indicator binding agents to bind to one or more pathogen indicators associated with one or more samples to form one or more magnetically active pathogen indicator complexes and separate the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprise:

one or more magnetic separation fluids.

39. The system of claim 35, further comprising:

one or more detection units configured to detect the one or more pathogen indicators associated with the one or more samples.

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65. A system comprising:

one or more microfluidic chips that are configured to allow one or more magnetically active pathogen indicator binding agents to bind to one or more pathogen indicators associated with one or more samples to form one or more magnetically active pathogen indicator complexes and separate the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially antiparallel flow with the one or more samples.

66. The system of claim 65, wherein the one or more microfluidic chips that are configured to allow one or more magnetically active pathogen indicator binding agents to bind to one or more pathogen indicators associated with one or more samples to form one or more magnetically active pathogen indicator complexes and separate the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially antiparallel flow with the one or more samples comprise:

one or more magnetic separation fluids.

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69. The system of caim 65, further comprising:

one or more detection units configured to detect the one or more pathogen indicators associated with the one or more samples.

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