IN VIVO BIOREACTORS AND METHODS OF MAKING AND USING SAME
First Claim
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1. A method for promoting generation of cartilage tissue, comprising administering in or adjacent to periosteum tissue of a subject a therapeutically effective amount of a biocompatible hydrogel comprising at least one polymer, wherein exogenous cells are substantially absent, wherein non-carbohydrate anti-angiogenic agents and/or growth factors are substantially absent.
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Abstract
The present invention relates to an in vivo method of promoting the growth of autologous cartilage and bone tissue, including tissue that can be explanted to other locations in the subject.
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Citations
25 Claims
- 1. A method for promoting generation of cartilage tissue, comprising administering in or adjacent to periosteum tissue of a subject a therapeutically effective amount of a biocompatible hydrogel comprising at least one polymer, wherein exogenous cells are substantially absent, wherein non-carbohydrate anti-angiogenic agents and/or growth factors are substantially absent.
- 14. A method for promoting generation of cartilage tissue, comprising administering in or adjacent to periosteum tissue of a subject a therapeutically effective amount of a biocompatible hydrogel consisting essentially of agarose and water.
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19. A method for promoting generation of cartilage tissue, comprising the steps of:
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(a) creating an artificial space or environment in an organ or cavity of a subject; (b) administering in or adjacent to periosteum tissue of the subject a therapeutically effective amount of a biocompatible hydrogel, thereby providing a hypoxic environment in the space; and (c) optionally, harvesting pluripotent or multipotent progenitor cells, chondrocytes, or cartilage from the artificial space or environment after the administration step.
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20. A kit for promoting generation of tissue in vivo, comprising a biocompatible hydrogel and two or more of:
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(a) a tissue retractor for generating an artificial space at a site in a subject; (b) an agent to partially degrade connective tissue at the site, thereby freeing cells to promote formation of the space and/or promote migration of cells into the space; (c) means for warming the hydrogel to a melting temperature; and (d) means for delivery of the melted gel to the site. - View Dependent Claims (21, 22, 23, 24, 25)
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Specification