RAPID MICROBIAL DETECTION AND ANTIMICROBIAL SUSCEPTIBIILITY TESTING

• US 20080241858A1
• Filed: 01/28/2008
• Published: 10/02/2008
• Est. Priority Date: 07/12/2003
• Status: Active Grant

• Current Assignee: Accelerate Diagnostics, Inc.
• Original Assignee: Accelr8 Technology Corporation
• Inventors: Goldberg, David A., Buttry, Daniel A., Howson, David C., Metzger, Steven W.
• Granted Patent: US 8,071,319 B2
• Time in Patent Office: Days
• US Class Current: 435/7.2
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 20/00 Nanooptics, e.g. quantum optics or photonic crystals
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/18 Testing for antimicrobial activity of a material
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/561 Immunoelectrophoresis

Abstract

A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms.

Claims

1. A method for measuring the growth of a plurality of individual microorganisms in a sample comprising:

contacting said sample with a biosensor comprising;

a concentration module;

at least one detection surface; and

concentrating said individual microorganisms onto said detection surface, wherein each said individual microorganism binds to said detection surface in spatially discrete sites;

allowing said individual microorganisms to grow for a first period of time; and

detecting growth of individual microorganisms as an indication of the presence of said microorganisms.