×

Defined media for pluripotent stem cell culture

  • US 20080241919A1
  • Filed: 10/29/2007
  • Published: 10/02/2008
  • Est. Priority Date: 12/31/2003
  • Status: Abandoned Application
First Claim
Patent Images

1. A system for culturing mammalian primordial stem cells in a substantially undifferentiated state, comprising:

  • a. a defined, isotonic culture medium that is essentially feeder-free and serum-free, comprising;

    (i) a basal medium;

    (ii) an amount of bFGF sufficient to support growth of substantially undifferentiated mammalian stem cells;

    (iii) an amount of insulin sufficient to support growth of substantially undifferentiated mammalian stem cells; and

    (iv) an amount of ascorbic acid sufficient to support growth of substantially undifferentiated mammalian stem cells;

    b. a cell culture vessel that includes a substrate comprising a cell-free matrix; and

    c. a population of mammalian primordial stem cells to be cultured in a substantially undifferentiated state.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×