Methods for pathogen detection

• US 20080241935A1
• Filed: 03/27/2007
• Published: 10/02/2008
• Est. Priority Date: 03/27/2007
• Status: Abandoned Application

• Current Assignee: Searete LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC (Intellectual Ventures LLC)
• Inventors: Lord, Robert W., Jung, Edward K.Y., Levien, Royce A., Malamud, Mark A., Leuthardt, Eric C., Rinaldo, John D., Wood, Lowell L.
• Application Number:
  US11/729,301
• Publication Number:
  US 20080241935A1
• Time in Patent Office: Days
• US Class Current: 436/20
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 2030/381 centrifugal chromatography
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The present disclosure relates to methods, systems, devices, and microfluidic chips that may be used for the detection of pathogens.

Claims

1-18. -18. (canceled)

19. A method comprising:

combining one or more samples with one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes; and

separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples.

20. The method of claim 19, wherein the combining one or more samples with one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes comprises:

combining the one or more samples with at least one magnetically active antibody, aptamer, polynucleotide, or polypeptide.

21. The method of claim 19, wherein the separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

separating the one or more magnetically active pathogen indicator complexes through use of magnetic attraction or magnetic repulsion.

22. The method of claim 19, wherein the separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

separating the one or more magnetically active pathogen indicator complexes through use of one or more ferrofluids.

23. The method of claim 19, further comprising:

analyzing the one or more samples with one or more analysis units.

25. The method of claim 23, further comprising:

identifying one or more pathogens present within the one or more samples.

24. (canceled)

26-36. -36. (canceled)

37. A method comprising:

accepting one or more samples that include one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes; and

separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples.

41. The method of claim 37, wherein the accepting one or more samples that include one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes comprises:

accepting the one or more samples that include one or more food products.

42. The method of claim 37, wherein the accepting one or more samples that include one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes comprises:

accepting the one or more samples that include one or more biological samples.

43. The method of claim 37, wherein the separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

separating the one or more magnetically active pathogen indicator complexes through use of magnetic attraction or magnetic repulsion.

44. The method of claim 37, wherein the separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

separating the one or more magnetically active pathogen indicator complexes through use of one or more ferrofluids.

45. The method of claim 37, further comprising:

analyzing the one or more samples with one or more analysis units.

47. The method of claim 45, further comprising:

identifying one or more pathogens present within the one or more samples.

49. The method of claim 47, wherein the identifying one or more pathogens present within the one or more samples comprises:

displaying an identity of the one or more pathogens present within the one or more samples.

38-40. -40. (canceled)

46. (canceled)

48. (canceled)

50-62. -62. (canceled)

63. A method comprising:

separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples.

64. The method of claim 63, wherein the separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

separating the one or more magnetically active pathogen indicator complexes through use of magnetic attraction or magnetic repulsion.

65. The method of claim 63, wherein the separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

separating the one or more magnetically active pathogen indicator complexes through use of one or more ferrofluids.

66. The method of claim 63, further comprising:

detecting one or more pathogen indicators with one or more detection units.

68. The method of claim 66, further comprising:

identifying one or more pathogens present within the one or more samples.

70. The method of claim 68, wherein the identifying one or more pathogens present within the one or more samples comprises:

displaying an identity of the one or more pathogens present within the one or more samples.

67. (canceled)

69. (canceled)

71-78. -78. (canceled)