Methods And Compositions For Grafting Functional Loops Into A Protein
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Abstract
The present invention provides targeted enzymes that bind to targets better than the corresponding pre-targeted enzymes bind the target under like conditions, methods of making targeted enzymes, methods of using targeted enzymes to treat diseases, and pharmaceutical compositions comprising targeted enzymes.
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9 Claims
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1. (canceled)
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2. (canceled)
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3. (canceled)
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4. A method of making a targeted enzyme, comprising
a) selecting a modified enzyme having a measurable enzymatic activity from a library of modified enzymes, said library comprising modified enzymes that have an inserted peptide that a corresponding unmodified enzyme does not have, and said unmodified enzyme having the measurable enzymatic activity, and b) replacing the inserted peptide in the selected modified enzyme with a binding peptide that binds a target, wherein the enzyme with the binding peptide has the measurable enzymatic activity and binds the target.
Specification