Adhesive Bead For Immobilization of Biomolecules and Method For Fabricating a Biochip Using the Same
First Claim
1. An adhesive bead functioning both as a solid support immobilizing biomolecules and as an adhesive adhering to the surface of a chip substrate, which is prepared by a method comprises:
- emulsifying a hydrophilic monomer, a mainmonomer and a comonomer in an aqueous medium; and
polymerizing the aqueous emulsion.
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Abstract
The present invention relates to an adhesive bead for immobilizing biomolecules and a method for fabricating a biochip using the same, and more particularly, relates to an adhesive bead functioning both as a solid support immobilizing biomolecules and an adhesive to the surface of a biochip substrate, and a method for fabricating a biochip, the method comprising the steps of immobilizing biomolecules to the adhesive bead to prepare an aqueous suspension of beads on which biomolecules are fixed and fixing the aqueous suspension on a substrate. The adhesive beads of the present invention can be directely immobilized on a biochip without additional equipment and treatment process due to the dual functions of a solid support immobilizing biomolecules and an adhesive to the surface of a substrate.
14 Citations
23 Claims
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1. An adhesive bead functioning both as a solid support immobilizing biomolecules and as an adhesive adhering to the surface of a chip substrate, which is prepared by a method comprises:
- emulsifying a hydrophilic monomer, a mainmonomer and a comonomer in an aqueous medium; and
polymerizing the aqueous emulsion. - View Dependent Claims (2, 3, 4, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- emulsifying a hydrophilic monomer, a mainmonomer and a comonomer in an aqueous medium; and
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5. A method for preparing an adhesive bead, the method comprises:
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(a) obtaining an emulsion by adding monomer(s) to an aqueous solution of an emulsifying agent; (b) stirring the mixture of said emulsion obtained in the step (a) and a solution which is prepared with hydrophilic monomer(s) in an aqueous medium and then heated up to about 75°
C. in N2 atmosphere; and(c) carrying out polymerization by adding a polymerizing initiator to the emulsion obtained in the step (b). - View Dependent Claims (6, 7, 8, 9, 10, 11)
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Specification