DNA Polymerase Blends and Mutant DNA Polymerases

• US 20080254525A1
• Filed: 03/20/2008
• Published: 10/16/2008
• Est. Priority Date: 06/28/2005
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Invitrogen Corp. (Thermo Fisher Scientific Incorporated)
• Inventors: Lee, Jun E., Potter, Robert Jason, Mandelman, David, Zheng, Weidong
• Granted Patent: US 9,376,698 B2
• Time in Patent Office: Days
• US Class Current: 435/193
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12N 9/1276 RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
• CPC Class Codes: C12P 19/34 Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2521/101 DNA polymerase
• CPC Class Codes: C12Q 2527/149 Concentration of an enzyme
• CPC Class Codes: C12Q 2561/101 Taqman
• CPC Class Codes: C12Y 207/07007 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: Y02P 20/52 using catalysts, e.g. selective catalysts

Abstract

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,

Claims

1-3. -3. (canceled)

4. An isolated mutant Tfi DNA polymerase having a D144A point mutation.

5. An isolated mutant Tfi DNA polymerase having D144A and E437D point mutations.

6-21. -21. (canceled)