Surfaces differentially adhesive to bacteria and eukaryotic cells

• US 20080255677A1
• Filed: 04/08/2008
• Published: 10/16/2008
• Est. Priority Date: 04/10/2007
• Status: Active Grant

• Current Assignee: The Trustees of The Stevens Institute of Technology (Stevens Institute Of Technology)
• Original Assignee: The Trustees of The Stevens Institute of Technology (Stevens Institute Of Technology)
• Inventors: Libera, Matthew R.
• Granted Patent: US 8,093,039 B2
• Time in Patent Office: Days
• US Class Current: 623/23.57
• CPC Class Codes: A61F 2/30767 Special external or bone-contacting surface, e.g. coating for improving bone ingrowth
• CPC Class Codes: A61F 2/3094 Designing or manufacturing processes
• CPC Class Codes: A61F 2002/0086 for preferentially controlling or promoting the growth of specific types of cells or tissues of bone tissue onto implantable joints A61F2002/3093
• CPC Class Codes: A61F 2002/30028 differing in tissue ingrowth capacity, e.g. made from both ingrowth-promoting and ingrowth-preventing parts
• CPC Class Codes: A61F 2002/3084 Nanostructures
• CPC Class Codes: A61F 2002/3093 for promoting ingrowth of bone tissue
• CPC Class Codes: A61F 2250/0051 differing in tissue ingrowth capacity, e.g. made from both ingrowth-promoting and ingrowth-preventing parts
• CPC Class Codes: A61F 2310/00598 Coating or prosthesis-covering structure made of compounds based on metal oxides or hydroxides
• CPC Class Codes: A61L 2400/18 Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/50 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: Y10S 530/815 Carrier is a synthetic polymer

Abstract

A surface coating comprises a primer coat that permits adhesion of eukaryotic cells thereto, and a plurality of macromolecular structures attached to the primer coat. At least some of the macromolecular structures have a cell-resistant character, meaning that cells generally will not adhere to them. The macromolecular structures are distributed across an area of the primer coat so that the surface coating permits adhesion of the eukaryotic cells to the primer layer and resists the adhesion of non-eukaryotic cells. Typically, the primer coat comprises a self-assembled polymeric monolayer and the macromolecular structures comprise nanoscale hydrogels. Such surface coatings may be formed on articles of manufacture for insertion into the body, such as orthopedic devices.

Claims

1. A surface coating, comprising:

a primer coat that permits adhesion of eukaryotic cells thereto; and

a plurality of macromolecular structures attached to a surface of said primer coat and exposed at said surface, wherein each of said plurality of macromolecular structures has at least two submicron dimensions, and wherein at least some of said plurality of macromolecular structures have a cell-resistant character, said macromolecular structures having a cell-resistant character being sufficiently distributed across an area of said primer coat so as to permit adhesion of eukaryotic cells thereto and resist the adhesion of non-eukaryotic cells thereto.

2. The surface coating of claim 1, wherein another at least some others of said plurality of macromolecular structures include a cell-adhesion factor that promotes adhesion of eukaryotic cells to said some others of said plurality of macromolecular structures.

3. The surface coating of claim 2, wherein the ratio of the number of said macromolecular structures including said cell-adhesion factor to the number of macromolecular structures having said cell-resistant character is in the range of from about one-to-one to about ten-to-one.

4. The surface coating of claim 1, wherein said primer coat includes a cell-adhesion factor that promotes adhesion of eukaryotic cells to said primer coat.

5. The surface coating of claim 4, wherein said cell-adhesion factor promotes specific adhesion of eukaryotic cells to said primer coat.

6. The surface coating of claim 1, wherein said macromolecular structures having a cell-resistant character also have an average spacing one from another that is sufficient to permit eukaryotic cells to proliferate among said macromolecular structures having a cell-resistant character.

7. The surface coating of claim 6, wherein said average spacing is in the range of about 0.2 μ

m to about 5 μ

m.

8. The surface coating of claim 1, wherein each of said at least some of said macromolecular structures comprises a hydrogel.

9. The surface coating of claim 1, wherein each of said plurality of said macromolecular structures are hydrogels.

10. The surface coating of claim 1, wherein at least one of said at least two submicron dimensions is in the range of about 50 nm to about 250 nm.

11. The surface coating of claim 1, wherein said macromolecular structures having a cell-resistant character occupy from about 2 percent to about 20 percent of said primer coat.

12. The surface coating of claim 1, wherein said primer coat includes at least one layer comprising a polymer.

13. The surface coating of claim 12, wherein said layer is a self-assembled monolayer of said polymer.

14. The surface coating of claim 13, wherein said self-assembled monolayer of said polymer has been formed by a process of electrostatic deposition of said polymer on a substrate.

15. The surface coating of claim 14, wherein at least some of said plurality of macromolecular structures have been deposited on said primer coat by electrostatic self-assembly.

16. An article, at least a portion of said article being a substrate having a surface, said article having a surface coating comprising a primer coat that permits adhesion of eukaryotic cells thereto and has been formed on said surface of said substrate, and a plurality of macromolecular structures attached to a surface of said primer coat and exposed at said surface of said primer coat, wherein each of said plurality of macromolecular structures has at least two submicron dimensions, and wherein at least some of said plurality of macromolecular structures have a cell-resistant character, said macromolecular structures having a cell-resistant character being sufficiently distributed across an area of said primer coat so as to permit adhesion of eukaryotic cells thereto and resist the adhesion of non-eukaryotic cells thereto.

17. The article of claim 16, wherein said surface of said substrate is topographically complex, said surface coating covering at least a portion of said surface of said substrate.

18. The article of claim 17, wherein said surface of said primer coat comprises a self-assembled monolayer of a polymer deposited onto said surface of said substrate.

19. The article of claim 18, wherein said article is selected to be inserted into a living body.

20. The article of claim 19, wherein said article is an orthopedic implant.