Biosensor

• US 20080257725A1
• Filed: 01/31/2008
• Published: 10/23/2008
• Est. Priority Date: 09/12/1997
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Inventors: Mure, Hiroki, Shirakawa, Hiroshi, Gotoh, Masao
• Granted Patent: US 7,918,988 B2
• Time in Patent Office: Days
• US Class Current: 204/403.01
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

Claims

1. A biosensor which comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially adhered by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

2. A biosensor according to claim 1 wherein the working electrode and the counter electrode are formed one at a time on the upper and lower plates, respectively, so as to have a facing structure on the inside.

3. A biosensor according to claim 1 wherein an oxidoreductase-electron transmitter mixture layer is formed on the working electrode.

4. A biosensor according to claim 1 wherein the glucose oxidase is immobilized by means of application drying.

5. A biosensor according to claim 1 wherein the adhesive layer formed by a double-sided adhesive tape.

6. A biosensor according to claim 1 wherein one end side of each base forms a tapered part, and the tip part of the working electrode or counter electrode is provided on the tapered part.

7. A biosensor according to claim 1 wherein a spacer with adhesive on both sides is interposed between the base having the working electrode arranged thereon and the base having the counter electrode arranged thereon.

8. A biosensor according to claim 1 wherein a reference electrode is arranged on the working electrode side or the counter electrode side.

9. A biosensor according to claim 1 wherein the adhesive layer is formed by the adhesive applied to one base.

10. A biosensor according to claim 1 wherein the adhesive layer is formed by the adhesives applied to both the bases.

11. A biosensor according to claim 1 wherein an insulating layer is formed between the electrode-formed base and the adhesive layer.

12. A biosensor according to claim 1 wherein one end side of each base is formed into a protruding shape.

13. A biosensor according to claim 1 wherein an oxidoreductase is immobilized also on the counter electrode.

14. A biosensor which comprises an oxidoreductase immobilized on an electrode, a working electrode and a counter electrode formed on the inside of a longer lower base and a shorter upper base through an adhesive layer so as to have a facing structure, and lead part for each electrodes formed in a position of the lower base where the end part is never superposed on the upper base, the electrode on the upper base being conducted to its lead part through the adhesive layer.

16. A biosensor according to claim 14 wherein the continuity through the adhesive layer is performed by a conductive material filled in the bored part of a double-sided adhesive tape.

17. A biosensor according to claim 14 wherein the continuity through the adhesive layer is performed by a conductive material filled in the bored part of the adhesive layer applied to one base.

18. A biosensor according to claim 14 wherein the continuity through the adhesive layer is performed by a conductive material filled in the bored parts of the adhesive layers applied to both the bases.

23. A biosensor according to claim 14 wherein an oxidoreductase-electron transmitter mixture layer is formed on the working electrode.

24. A biosensor according to claim 14 wherein oxidoreductase-electron transmitter mixture layers are formed on the working electrode and the counter electrode.

25. A biosensor according to claim 14 which is further provided with a reference electrode.

26. A biosensor according to claim 14 wherein one end side of each base forms a tapered part, and the tip part of the working electrode or the counter electrode is provided on each tapered part.

15. A biosensor which comprises an oxidoreductase immobilized on an electrode, a working electrode and a counter electrode form on the inside of a longer upper base and shorter lower base through an adhesive layer so as to have a facing structure, and lead parts for these electrodes formed on the surface side of the upper base, respectively, the electrode provided on the upper base being conducted to its lead part through the base, and the electrode provided on the lower base being conducted to its lead part provided on the upper base through the adhesive layer.

19. A biosensor according to claim 15 wherein the continuity through the adhesive layer is performed by a conductive material filled in the bored part of the double-sided adhesive tape.

20. A biosensor according to claim 15 wherein the continuity through the adhesive layer is performed by a conductive material filled in the bored part of the adhesive layer applied onto one base.

21. A biosensor according to claim 15 wherein the continuity through the adhesive layer is performed by a conductive material filled in the bored parts of the adhesive layers applied onto both the bases.

22. A biosensor according to claim 15 wherein the continuity through the adhesive layer is performed by a conductive material filled in the bored part of the base.

27. A biosensor according to claim 15 wherein oxidoreductase-electron transmitter mixture layer is formed on the working electrode.

28. A biosensor according to claim 15 wherein oxidoreductase-electron transmitter mixture layers are formed on the working electrode and the counter electrode.

29. A biosensor according to claim 15 which is further provided with a reference electrode.

30. A biosensor according to claim 15 wherein one end side of each base forms a tapered part, and the tip part of the working electrode or the counter electrode is provided on each tapered part.

31. A biosensor which comprises an oxidoreductase immobilized on an electrode, a working electrode and a counter electrode formed on the inside of a longer lower base and a shorter upper base through a spacer so as to have a facing structure, and a lead part for each electrode formed in a position on the lower base where the end part is never superposed on the upper base, the electrode on the upper base being conducted to its lead part through a spacer.

33. A biosensor according to claim 31 wherein the continuity through the spacer is performed by a conductive material filled in the bored part of the spacer.

35. A biosensor according to claim 31 wherein one end side of each base forms a tapered part, and the tip part of the working electrode or the counter electrode is provided on each tapered part.

36. A biosensor according to claim 31 wherein an oxidoreductase-electron transmitter mixture layer is formed on the working electrode.

37. A biosensor according to claim 31 wherein oxidoreductase-electron transmitter mixture layers are formed on the working electrode and the counter electrode.

38. A biosensor according to claim 31 which is further provided with a reference electrode.

32. A biosensor which comprises an oxidoreductase immobilized on an electrode, a working electrode and a counter electrode formed on the inside of a longer upper base and a shorter lower base through a spacer so as to have a facing structure, respectively, and lead parts for the electrodes formed on the surface side of the upper base, respectively, the electrode provided on the upper base being conducted to its lead part through the base, and the electrode provided on the lower base conducted to its lead part provided on the upper base through the spacer.

34. A biosensor according to claim 32 wherein the continuity through the base is performed by a conductive material filled in the bored part of the base.

39. A biosensor according to claim 32 wherein the continuity through the spacer is performed by a conductive material filled in the bored part of the spacer.

40. A biosensor according to claim 32 wherein one end side of each base forms a tapered part, and the tip part of the working electrode or the counter electrode is provided on each tapered part.

41. A biosensor according to claim 32 wherein an oxidoreductase-electron transmitter mixture layer is formed on the working electrode.

42. A biosensor according to claim 32 wherein oxidoreductase-electron transmitter mixture layers are formed on the working electrode and the counter electrode.

43. A biosensor device which comprises an element reaction sensor member to be inserted to the connector part of a device body in such a manner as to be attachable and detachable, the element reaction sensor member having each output terminal of a working electrode and a counter electrode to be electrically connected to connector part-side corresponding input terminals by insertion, and an element reaction part formed on at least the working electrode of the working electrode and the counter electrode, the element reaction sensor member further having a sensor insertion judging electrode, and the connector part of the device body having two input terminals with which the sensor insertion judging electrode output terminal makes contact, so that the system of the device body is started by the contact with the two input terminals to judge the sensor insertion by a control part.

44. A biosensor device according to claim 43 wherein the control part judges the dropping of a material to be measured by a signal for laying the working electrode and the counter electrode into closed circuit by the dropping of the material to the element reaction part, starts to count the residual time up to a preset measurement end, and transmits the count signal to a display part provided on the device body to display it.

45. A biosensor device according to claim 43 wherein the working electrode and the counter electrode are formed within the same plane of the base which is the body of the element reaction sensor member.

46. A biosensor device according to claim 43 wherein the element reaction part is formed of a mixture layer of an oxidoreductase and an electron transmitter.

47. A biosensor device according to claim 43 wherein the material to be measured is dropped onto the element reaction part in the state where a voltage is applied between the working electrode and the counter electrode after a fixed time from the sensor insertion, and the element current value generated by the oxidation is taken out and measured.

48. A biosensor comprising:

two layers and at least one electrode positioned with respect to the two layers to provide a sample receiving space;

at least one protrusion or tab at the sample receiving space for aiding in delivery of a biological fluid sample;

a space for housing a sample and an opening is in communication with the space for housing the sample, wherein the space for the housing has a volume that is no more than 1.0 μ

L; and

an enzyme reaction layer having a thickness of about 1-200 μ

m in the sample receiving space,

wherein the biosensor is capable of analysis of a sample having a volume of no more than about 1.0 μ

L.