Method and Apparatus for Performing Biochemical Testing in a Microenvironment

• US 20080260589A1
• Filed: 06/30/2008
• Published: 10/23/2008
• Est. Priority Date: 10/11/2001
• Status: Abandoned Application

• Current Assignee: Lextron Systems, Inc.
• Original Assignee: Lextron Systems, Inc.
• Inventors: Kikinis, Dan
• Application Number:
  US12/164,626
• Publication Number:
  US 20080260589A1
• Time in Patent Office: Days
• US Class Current: 422/99
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 15/0255 with mechanical, e.g. inertial, classification, and investigation of sorted collections with centrifuges G01N15/042
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A micro-testing lab for performing tests on biochemical and synthetic materials is provided. The testing lab includes a substrate forming the base material of the test lab; a poly silicon layer formed over the substrate; and a silicon dioxide layer deposited over the poly silicon layer, the poly silicon layer supporting a series of grooves, flow obstacles, and sensors for facilitating material flow, material separation, and material analysis. In a preferred embodiment, material is prepared in a preparation basin and introduced into a groove and propelled there through to at least one flow obstacle separating different molecules of the material to be tested and wherein upon separation, at least one sensor is utilized for performing analysis of the material. Also in preferred embodiments, the lab is field programmable and controllable through a control interface.

Claims

1. A micro-testing lab for performing tests on biochemical and synthetic materials comprising:

a substrate forming the base material of the test lab;

a poly silicon layer formed over the substrate; and

a silicon dioxide layer deposited over the poly silicon layer, the poly silicon layer supporting a series of grooves, flow obstacles, and sensors for facilitating material flow, material separation, and material analysis;

characterized in that material is prepared in a preparation basin and introduced into a groove and propelled there through to at least one flow obstacle separating different molecules of the material to be tested and wherein upon separation, at least one sensor is utilized for performing analysis of the material.

2. The micro-testing lab of claim 1 wherein the substrate is a section of AM LCD manufactured glass.

3. The micro-testing lab of claim 1 wherein the substrate is a section of silicon wafer material.

4. The micro-testing lab of claim 1 wherein the substrate is a section of polymer material.

5. The micro-testing lab of claim 1 wherein the grooves are in the shape of a V.

6. The micro-testing lab of claim 1 wherein the flow obstacles comprise a series of zigzags in the groove path.

7. The micro-testing lab of claim 1 wherein the flow obstacles include a combination of zigzags, bottlenecks, and surface treatments.

8. The micro-testing lab of claim 7 wherein the surface treatment is an antigen for binding to certain molecules of the material and stopping forward progression of the bound molecules.

9. The micro-testing lab of claim 1 wherein material introduction is performed using inkjet technology.

10. The micro-testing lab of claim 1 wherein the material is propelled through the grooves by electrodes enabled to attract or repulse charged particles of the material.

11. The micro-testing lab of claim 1 wherein the at least one sensor is one of an electrostatic sensor, an electro-conductive sensor, an electro-dynamic sensor, a photo transmissive sensor, or a photo reflective sensor.

12. The micro-testing lab of claim 1 wherein there are a plurality of sensors, the sum total defining a combination of sensor types including an electrostatic sensor, an electro-conductive sensor, an electro-dynamic sensor, a photo transmissive sensor, and a photo reflective sensor.

13. The micro-testing lab of claim 1 further comprising at least one collector basin for temporarily collecting material at a collection point along a groove.

characterized in that the material is urged into the collector basin through at least one via opening from the groove to the basin.

14. The micro-testing lab of claim 13 wherein the material is exited out of the collector basin using inkjet technology.

15. The micro-testing lab of claim 10 further comprising at least one separation switch for urging material from a primary groove having access to a secondary groove into the secondary groove, the switch comprising:

a gatekeeper electrode for attracting charged particles into the secondary groove and,

a set of propulsion electrodes in the primary groove combining function with the gatekeeper electrode to divert material from the primary path to the secondary path.

16. The micro-testing lab of claim 15 wherein the material is diverted into a collector basin.

17. A field-programmable system for testing and analyzing biochemical and synthetic materials comprising:

a micro-testing lab having a substrate layer, a poly silicon layer and a silicon dioxide layer, the silicon dioxide layer including a series of grooves, flow obstacles, and sensors for facilitating material flow, material separation, and material analysis;

a microprocessor having line access to the sensors and to a distributed system of electrodes embedded along the grooves, the electrodes adapted to urge the material through the grooves;

a control-interface and display monitor having line access to the microprocessor for issuing commands to the processor related to programmable functions of the sensors and electrodes and for displaying test data; and

at least one peripheral device having line access to the microprocessor and to the control-interface, the at least one device adapted to function in cooperation with at last one sensor according to trigger states;

characterized in that a user operating the control-interface can program test criteria automate certain test procedures and compare test results in conjunction with a material test scenario conducted on the micro-testing lab.

18. The system of claim 17 wherein the microprocessor is embedded within the micro-testing lab.

19. The system claim 17 wherein the substrate layer is AM LCD manufactured glass.

20. The system of claim 17 wherein the substrate layer is silicon wafer material.

21. The system of claim 17 wherein the substrate layer is polymer material.

22. The system of claim 17 wherein the grooves are in the shape of a V.

23. The system of claim 17 wherein the flow obstacles comprise a series of zigzags in the groove path.

24. The system of claim 17 wherein the flow obstacles include a combination of zigzags, bottlenecks, and surface treatments.

25. The system of claim 24 wherein the surface treatment is an antigen for binding to certain molecules of the material and stopping forward progression of the bound molecules.

26. The system of claim 17 wherein material introduction into grooves is performed using inkjet technology.

27. The system of claim 17 wherein sensors include one or a combination of an electrostatic sensor, an electro-conductive sensor, an electro-dynamic sensor, a photo transmissive sensor, or a photo reflective sensor.

28. The system of claim 17 wherein the control-interface is a computer workstation.

29. The system of claim 17 wherein the at least one peripheral device is one of a UV laser, a particle counter, or a mass spectrometer.