Tagged Polyfunctional Reagents Capable of Reversibly Binding Target Substances in a pH-dependent Manner
First Claim
1. A soluble polyfunctional reagent which is capable at a first pH of binding to a target substance and is capable at a second pH of releasing the target substance, wherein the reagent further comprises a tagging group for manipulating or detecting the target substance when bound to the polyfunctional reagent.
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Accused Products
Abstract
Polyfunctional reagents are disclosed that are capable of reversibly binding to target substances, for example nucleic acid, proteins, polypeptides, cells, cell components, microorganisms or viruses, for use in purifying or otherwise manipulating them. The reagents comprise a tagging group for manipulating and/or detecting the target substance when bound to the polyfunctional reagent. The polyfunctional reagents work by binding the target substance at a first pH and then releasing it at a second pH, usually higher than the first. Examples of tagging groups include tagging group members of a specific binding pair which is capable of binding to a specific binding partner and/or a label.
56 Citations
33 Claims
- 1. A soluble polyfunctional reagent which is capable at a first pH of binding to a target substance and is capable at a second pH of releasing the target substance, wherein the reagent further comprises a tagging group for manipulating or detecting the target substance when bound to the polyfunctional reagent.
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33. A polyfunctional reagent which is capable at a first pH at which the reagent is positively charged of binding to a target substance and is capable at a second, higher pH at which the reagent is less positive, neutral or negatively charged of releasing the target substance, wherein the reagent further comprises a tagging group for manipulating or detecting the target substance when bound to the polyfunctional reagent and wherein the reagent has positively ionisable groups having a pKa between 4.5 and 8.5.
Specification