Compositions And Methods For Treating And Diagnosing Pancreatic Cancer
First Claim
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1. An isolated population of cancer stem cells that are:
- a) tumorigenic;
b) CD44+;
c) CD24+ and,d) ESA+.
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Abstract
The present invention relates to the field of oncology and provides novel compositions and methods for diagnosing and treating pancreatic cancer. In particular, the present invention provides pancreatic cancer stem cells useful for the study, diagnosis, and treatment of solid tumors.
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Citations
11 Claims
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1. An isolated population of cancer stem cells that are:
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a) tumorigenic; b) CD44+; c) CD24+ and, d) ESA+. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of obtaining from a tumor an isolated population of cancer stem cells comprising:
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a) contacting a mixture of tumor cells from a tumor with reagents against CD44, CD24, and ESA, and b) positively selecting for cells that are CD44+, CD24+, and ESA+.
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8. A method of identifying the presence of pancreatic cancer stem cells in a subject suspected of having cancer, wherein the method comprises:
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(a) obtaining a biological sample from the subject; (b) dissociating cells of the sample; (c) contacting the dissociated cells with a first reagent that binds CD44, a second reagent that binds CD24, and a third reagent that binds ESA; and (d) detecting cancer stem cells that bind to the first, second, and third reagents. - View Dependent Claims (9, 10)
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11. A method of treating a patient with pancreatic cancer comprising;
- administering a SHH inhibitor to said patient such that the number of pancreatic stem cells expressing CD44, CD24 and ESA is reduced or eliminated in the subject.
Specification