Procedure for the determination of the reaction lag phase in an analyte-dependent reaction
First Claim
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1. A method for determining the concentration or activity of an analyte in a sample, the method comprising:
- mixing a sample with at least one reagent, whereby an analyte-dependent reaction is set in motion;
measuring a signal (x) changing over time (t) as a result of the analyte-dependent reaction and storing a resultant signal-time curve;
determining a maximum increase in the signal-time curve;
determining a reaction lag phase of the signal-time curve; and
determining a concentration or activity of the analyte by means of at least one parameter of the signal-time curve, wherein the parameter occurs after the reaction lag phase in terms of time, andwherein determining the reaction lag phase includes determining a period of time from a time of a first signal (t0) up to a time tlag,bydetermining a time tmax of the maximum increase in the signal-time curve; and
subsequently determining from the time tmax, the time tlag, where tlag is smaller than tmax and where tlag corresponds to the time at which the increase in the signal-time curve for the first time falls short of a threshold value and where the threshold value corresponds to a test-specific, predetermined fraction of the maximum increase in the signal-time curve.
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Abstract
The invention is in the field of analytical technique and relates to an improved procedure for determining the concentration or activity of an analyte, an analyte-dependent detection reaction being set in motion and the reaction kinetics determined being evaluated. The procedure makes possible an individual determination of the lag phase of the reaction kinetics for each measurement.
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14 Claims
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1. A method for determining the concentration or activity of an analyte in a sample, the method comprising:
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mixing a sample with at least one reagent, whereby an analyte-dependent reaction is set in motion; measuring a signal (x) changing over time (t) as a result of the analyte-dependent reaction and storing a resultant signal-time curve; determining a maximum increase in the signal-time curve; determining a reaction lag phase of the signal-time curve; and determining a concentration or activity of the analyte by means of at least one parameter of the signal-time curve, wherein the parameter occurs after the reaction lag phase in terms of time, and wherein determining the reaction lag phase includes determining a period of time from a time of a first signal (t0) up to a time tlag, by determining a time tmax of the maximum increase in the signal-time curve; and subsequently determining from the time tmax, the time tlag, where tlag is smaller than tmax and where tlag corresponds to the time at which the increase in the signal-time curve for the first time falls short of a threshold value and where the threshold value corresponds to a test-specific, predetermined fraction of the maximum increase in the signal-time curve. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An apparatus for an automatic implementation of a method of determining the concentration or activity of an analyte in a sample, the apparatus comprising:
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means for measuring a signal (x) changing over time (t) as a result of an analyte-dependent reaction, means for storing a resultant signal-time curve, means for determining a time tmax of a maximum increase in the stored signal-time curve, and means for controlling implementation of a step for determining a time tlag. - View Dependent Claims (14)
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Specification