DISPOSABLE MICROPURIFICATION CARDS, METHODS, AND SYSTEMS THEREOF
First Claim
Patent Images
1. A micropurification card, comprising:
- a plurality of fluidic components capable of extracting molecules from a sample, the plurality of fluidic components substantially oriented in a plane, the plurality of fluidic components comprising;
a sample loading inlet,an elution inlet,a lysing region capable of being heated to at least about 90°
C. and pressurized to at least about 10 psi greater than the ambient atmospheric pressure to provide a lysed sample,a filter capable of filtering molecules from the lysed sample,a molecule capture region capable of being heated to at least about 40°
C., andan elution tip,wherein the sample loading inlet is in fluidic communication with the lysing region, the lysing region being in fluidic communication with the filter, the filter being capable of fluidically communicating one or more molecules to the molecule capture region, and the molecule capture region being in fluidic communication with both the elution inlet and the elution tip.
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Abstract
Disclosed herein are micropurification cards, systems, systems and methods, backspace. A micropurification cards include a plurality of fluidic components capable of extracting molecules from samples. Samples include biological cells and the extracted molecules include nucleic acids.
50 Citations
53 Claims
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1. A micropurification card, comprising:
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a plurality of fluidic components capable of extracting molecules from a sample, the plurality of fluidic components substantially oriented in a plane, the plurality of fluidic components comprising; a sample loading inlet, an elution inlet, a lysing region capable of being heated to at least about 90°
C. and pressurized to at least about 10 psi greater than the ambient atmospheric pressure to provide a lysed sample,a filter capable of filtering molecules from the lysed sample, a molecule capture region capable of being heated to at least about 40°
C., andan elution tip, wherein the sample loading inlet is in fluidic communication with the lysing region, the lysing region being in fluidic communication with the filter, the filter being capable of fluidically communicating one or more molecules to the molecule capture region, and the molecule capture region being in fluidic communication with both the elution inlet and the elution tip. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A disposable micropurification card, comprising:
an injection-molded card comprising a plurality of fluidic components capable of extracting molecules from a sample, the plurality of fluidic components comprising a sample loading inlet capable of being in fluidic communication with a lysing region, the lysing region being in fluidic communication with a filter, the filter being capable of fluidically communicating one or more molecules to a molecule capture region, and the molecular capture region being in fluidic communication with an elution inlet and an elution tip. - View Dependent Claims (27, 28, 29)
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30. A system suitable for preparing samples in a micropurification card at elevated temperatures and pressures, the system comprising:
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a sample input fluid connection capable of being fluidically connected under pressure to a sample loading inlet on the micropurification card; an elution input fluid connection capable of being fluidically connected under pressure to a sample loading inlet to an elution inlet on the micropurification card; a card holder capable of positionally holding the micropurification card to receive said sample input fluid and elution input fluid connections, the card holder comprising; a heater capable of heating a lysing region on the micropurification card to at least about 90°
C.; anda thermal controller capable of heating a molecule capture region on the micropurification card to above about 40°
C. and cooling the molecule capture region to below about 30°
C.; and
;a positionable fluid collection holder capable of alternately positioning two or more collection fluid receptacles for receiving fluid exiting an elution tip on the micropurification card. - View Dependent Claims (31, 32, 33, 34)
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35. A system suitable for collecting molecules from samples using one or more disposable micropurification cards at elevated temperatures and pressures, the system comprising:
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one or more disposable micropurification cards, comprising;
an injection-molded card comprising a plurality of fluidic components capable of extracting molecules from a sample comprising one or more cells, the plurality of fluidic components comprising a sample loading inlet capable of being in fluidic communication with a lysing region, the lysing region being in fluidic communication with a filter, the filter being capable of fluidically communicating one or more molecules to a molecule capture region, and the molecular capture region being in fluidic communication with an elution inlet and an elution tip; anda system suitable for preparing samples in a micropurification card, the system comprising;
a sample input fluid connection capable of being fluidically connected under pressure to the sample loading inlet on the micropurification card;an elution input fluid connection capable of being fluidically connected under pressure to a sample loading inlet to the elution inlet on the micropurification card; a card holder capable of holding the micropurification card in position to receive said sample input fluid and elution input fluid connections, the card holder comprising; a heater capable of heating a lysing region on the micropurification card to at least about 90°
C.; anda thermal controller capable of heating a molecule capture region on the micropurification card to above about 40°
C. and cooling the molecule capture region to below about 30°
C.; and
;a positionable fluid collection holder capable of receiving an elutant fluid comprising the molecules emanating from said elution tip. - View Dependent Claims (36, 37)
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38. A method of collecting molecules using a card-based sample preparation system, the method comprising:
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fluidically communicating a sample comprising cells and a first buffer solution under pressure from a sample loading inlet to a lysing region on a micropurification card; heating the sample in the lysing region to a temperature in the range of from about 100°
C. to about 150°
C. at one or more pressures greater than ambient pressure to lyse the cells to give rise to lysed cell fragments and molecules;filtering the molecules from the lysed cell fragments at a temperature greater than about 90°
C.;capturing at least a portion of the filtered molecules using a molecular capture material or device; eluting at least a portion of the captured molecules using a second buffer solution through an elution tip, the second buffer solution being the same or different than the first buffer solution; and collecting at least a portion of the eluted molecules and second buffer solution in a positionable fluid collection holder. - View Dependent Claims (39, 40, 52, 53)
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41. A method of collecting molecules using a card-based sample preparation system, the method comprising:
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fluidically communicating a sample under pressure from a sample loading inlet to a heating region on a micropurification card; heating the sample in the heater region to a temperature in the range of from about 100°
C. to about 150°
C. at one or more pressures greater than ambient pressure to breakdown at least a portion of the sample;filtering the molecules from sample fragments at a temperature greater than about 90°
C.;capturing at least a portion of the filtered molecules using a molecular capture material or device; eluting at least a portion of the captured molecules through an elution tip; and collecting at least a portion of the eluted molecules in a positionable fluid collection holder. - View Dependent Claims (42, 43, 44)
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45. A method for collecting molecules from lysed cells using a card-based sample preparation system, the method comprising:
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fluidically communicating a sample comprising cells and a first buffer solution under pressure from a sample loading inlet to a lysing region on a micropurification card; lysing the cells in the lysing region using a lysing agent at one or more pressures greater than ambient pressure to give rise to lysed cell fragments and molecules; filtering at least a portion of the molecules from the lysed cell fragments; capturing at least a portion of the filtered molecules using a molecular capture material or device; eluting at least a portion of the captured molecules using a second buffer solution through an elution tip, the second buffer solution being the same or different than the first buffer solution; and collecting at least a portion of the eluted molecules and second buffer solution in a positionable fluid collection holder. - View Dependent Claims (46, 47, 48, 49, 50, 51)
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Specification