COMBINATION READER
First Claim
Patent Images
1. An imaging system for collecting images of signals associated with a sample tile, the imaging system comprising:
- a stage supporting the sample tile;
a ring illuminator system emitting a uniform excitation energy upon an entirety of the sample tile causing at least a first signal to be generated from the sample tile; and
an image collecting device collecting a first image of the first signal, the image collecting device further collecting a second image of a second signal emitted from the sample tile, the second signal being different than the first signal.
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Abstract
An imaging system for collecting images of signals associated with a sample tile comprising a stage supporting the sample tile, a ring illuminator system emitting a uniform excitation energy upon an entirety of the sample tile causing at least a first signal to be generated from the sample tile, and an image collecting device collecting a first image of the first signal. The image collecting device further collecting a second image of a second signal emitted from the sample tile, wherein the second signal being different than the first signal.
100 Citations
20 Claims
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1. An imaging system for collecting images of signals associated with a sample tile, the imaging system comprising:
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a stage supporting the sample tile; a ring illuminator system emitting a uniform excitation energy upon an entirety of the sample tile causing at least a first signal to be generated from the sample tile; and an image collecting device collecting a first image of the first signal, the image collecting device further collecting a second image of a second signal emitted from the sample tile, the second signal being different than the first signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for collecting images of emissions using an imaging apparatus, the method comprising:
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placing a sample tile on a stage of the imaging apparatus, the sample tile having an array including a plurality of features, at least a portion of the features having a fluorophore; flooding an entirety of the sample tile with uniform-field light utilizing an ring illuminator of the imaging apparatus, the uniform-field light exciting the fluorophore in the array to output an emission; and collecting images of at least a portion of the emission utilizing an image collecting device of the imaging apparatus. - View Dependent Claims (12, 13, 14, 15, 16, 17)
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18. A method for collecting images of emissions using an imaging apparatus, the method comprising:
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placing a sample tile on a stage of the imaging apparatus, the sample tile having an array including a plurality of features, at least a portion of the features having a fluorescent marker; flooding an entirety of the sample tile with light defining a uniform-field utilizing an ring illuminator of the imaging apparatus, the uniform-field light exciting the fluorescent markers in the array; collecting images of at least a portion of the array utilizing an image collecting device of the imaging apparatus, the images selectively showing fluorescent signals emitted by the fluorescent markers when the ring illuminator floods the array with light; and actively focusing the image collecting device relative to the sample tile simultaneously with the collecting images of at least a portion of the array. - View Dependent Claims (19, 20)
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Specification