Multiphoton-excitation laser scanning microscope
First Claim
1. A multiphoton-excitation laser scanning microscope comprising:
- a multiphoton-excitation laser light source configured to emit ultrashort pulsed laser light;
a light-scanning unit configured to scan the ultrashort pulsed laser light emitted from the multiphoton-excitation laser light source in two dimensions;
an objective lens configured to focus the ultrashort pulsed laser light scanned by the light-scanning unit on the specimen;
a collector lens disposed opposite the objective lens, with the specimen disposed therebetween, to collect fluorescence emitted from the specimen as a result of multiphoton excitation; and
a light detector configured to detect the fluorescence collected by the collector lens;
wherein the collector lens has a higher numerical aperture and a larger field number than the objective lens.
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0 Petitions
Accused Products
Abstract
A multiphoton-excitation laser scanning microscope capable of efficiently collecting fluorescence emitted from a specimen to acquire a brighter multiphoton-excitation fluorescence image is provided. This multiphoton-excitation laser scanning microscope includes a multiphoton-excitation laser light source for emitting ultrashort pulsed laser light, a light-scanning unit configured to scan a specimen with the ultrashort pulsed laser light emitted from the multiphoton-excitation laser light source in two dimensions, an objective lens configured to focus the ultrashort pulsed laser light scanned by the light-scanning unit on the specimen, a collector lens disposed opposite the objective lens, with the light-scanning unit disposed therebetween, to collect fluorescence emitted from the specimen, and a light detector configured to detect the fluorescence collected by the collector lens. The collector lens has a higher numerical aperture and a larger field number than the objective lens.
9 Citations
14 Claims
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1. A multiphoton-excitation laser scanning microscope comprising:
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a multiphoton-excitation laser light source configured to emit ultrashort pulsed laser light; a light-scanning unit configured to scan the ultrashort pulsed laser light emitted from the multiphoton-excitation laser light source in two dimensions; an objective lens configured to focus the ultrashort pulsed laser light scanned by the light-scanning unit on the specimen; a collector lens disposed opposite the objective lens, with the specimen disposed therebetween, to collect fluorescence emitted from the specimen as a result of multiphoton excitation; and a light detector configured to detect the fluorescence collected by the collector lens; wherein the collector lens has a higher numerical aperture and a larger field number than the objective lens. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification