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Multiphoton-excitation laser scanning microscope

  • US 20080266551A1
  • Filed: 04/21/2008
  • Published: 10/30/2008
  • Est. Priority Date: 04/26/2007
  • Status: Active Grant
First Claim
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1. A multiphoton-excitation laser scanning microscope comprising:

  • a multiphoton-excitation laser light source configured to emit ultrashort pulsed laser light;

    a light-scanning unit configured to scan the ultrashort pulsed laser light emitted from the multiphoton-excitation laser light source in two dimensions;

    an objective lens configured to focus the ultrashort pulsed laser light scanned by the light-scanning unit on the specimen;

    a collector lens disposed opposite the objective lens, with the specimen disposed therebetween, to collect fluorescence emitted from the specimen as a result of multiphoton excitation; and

    a light detector configured to detect the fluorescence collected by the collector lens;

    wherein the collector lens has a higher numerical aperture and a larger field number than the objective lens.

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