Adamts-7 as a Biomarker for Cancers of Epithelial Origin

• US 20080268473A1
• Filed: 02/13/2006
• Published: 10/30/2008
• Est. Priority Date: 02/17/2005
• Status: Abandoned Application

• Current Assignee: Children's Medical Center Corporation
• Original Assignee: Children's Medical Center Corporation
• Inventors: Moses, Marsha A., Roy, Roopali
• Application Number:
  US11/815,821
• Publication Number:
  US 20080268473A1
• Time in Patent Office: Days
• US Class Current: 435/7.23
• CPC Class Codes: G01N 2333/96486 Metalloendopeptidases (3.4.24)
• CPC Class Codes: G01N 33/57407 Specifically defined cancers
• CPC Class Codes: G01N 33/57496 involving intracellular compounds

Abstract

ADAMTS-7 expression and activity are up regulated in patients that have cancers of epithelial origin. Accordingly, the present invention is directed to methods diagnosis of cancers of epithelial origin (e.g. breast cancer, prostate cancer, bladder cancer, brain cancer and hepatic cancer). In particular, the presence of ADAMTS-7 in a biological sample is indicative of cancer of epithelial origin. Thus, measuring the level of ADAMTS-7 in biological samples (e.g. urine or blood) provides a quick, easy, and safe screen that can be used to diagnose cancer in a patient.

Claims

1. A method for facilitating the diagnosis of a patient for a cancer of epithelial origin comprising:

a. obtaining a biological sample from the patient; and

b. detecting the presence or absence of ADAMTS-7 in the biological sample,

wherein the presence of ADAMTS-7 is indicative of the presence of cancer of epithelial origin.

2. The method of claim 1, wherein said biological sample is selected from the group consisting of blood, tissue, serum, urine, stool, sputum, cerebrospinal fluid, nipple aspirates, and supernatant from cell lysate.

3. The method of claim 1, wherein said biological sample is urine

7. The method of claim 1 or 4, wherein the cancer of epithelial origin is selected from the group consisting of breast cancer, basal cell carcinoma, adenocarcinoma, gastrointestinal cancer, lip cancer, mouth cancer, esophageal cancer, small bowel cancer, stomach cancer, colon cancer, liver cancer, brain, bladder cancer, pancreas cancer, ovary cancer, cervical cancer, lung cancer, skin cancer, prostate cancer, and renal cell carcinoma.

8. The method of claim 1, wherein the presence or absence of ADAMTS-7 is detected using an antibody-based binding moiety which specifically binds ADAMTS-7 protein.

12. The method according to claim 8 or 9, wherein the antibody-based binding moiety is labeled with a detectable label.

13. The method according to claim 12, wherein the label is selected from the group consisting of a radioactive label, a hapten label, a fluorescent label, and an enzymatic label.

14. The method according to claim 8 or 9, wherein the antibody-based binding moiety is an antibody.

15. The method according to claim 14, wherein the antibody is an monoclonal antibody.

4. A method for diagnosing cancer of epithelial origin in a patient comprising:

a. measuring ADAMTS-7 levels present in a test sample obtained from the patient;

b. comparing the level of ADAMTS-7 in the test sample with the level of ADAMTS-7 present in a control sample;

wherein a higher level of ADAMTS-7 in the test sample as compared to the level of ADAMTS-7 in the control sample is indicative of cancer of epithelial origin.

5. The method of claim 4, wherein said test sample and said control sample are selected from the group consisting of blood, tissue, serum, urine, stool, sputum, cerebrospinal fluid, nipple aspirates, and supernatant from cell lysate.

6. The method of claim 4, wherein said test and control samples are urine.

9. The of claim 4, wherein the level of ADAMTS-7 is measured by measuring the level of ADAMTS-7 protein.

10. The method of claim 4, wherein the level of ADAMTS-7 is measured by measuring the activity of ADAMTS-7.

11. The method of claim 9, wherein the level of ADAMTS-7 protein is measured by a method comprising the steps of:

a. contacting the test sample, or preparation thereof, with an antibody-based binding moiety which specifically binds ADAMTS-7 to form an antibody-ADAMTS-7 complex; and

b. detecting the presence of the complex, thereby measuring the level of ADAMTS-7 present.

16. A kit for detecting ADAMTS-7 in a urine sample comprising a container for holding a urine sample, and at least one antibody that specifically binds ADAMTS-7.

18. The kit of claim 18, further comprising directions for use.

17. The kit of claim 18, wherein the kit comprises two antibodies that specifically bind to ADAMTS-7, one antibody is immobilized on a solid phase and one antibody is detectably labeled.

19. A method to direct treatment of a subject which comprises having a subject tested for the presence of ADAMTS-7 in a biological sample obtained from the subject, wherein a clinician reviews the results and if the biological sample is positive for the presence of ADAMTS-7 the clinician directs the subject to be treated for cancer of epithelial origin.

20. The method of claim 19, wherein the biological sample is urine.