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Method for creating polynucleotide and polypeptide sequences

  • US 20080268505A1
  • Filed: 12/07/2006
  • Published: 10/30/2008
  • Est. Priority Date: 12/08/1997
  • Status: Active Grant
First Claim
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1. A method for evolving a polynucleotide toward acquisition of a desired property, comprising(a) incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes;

  • (b) exposing the heteroduplexes to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants;

    (c) screening or selecting the recombined polynucleotide variants for the desired property.

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