MOLECULARLY-IMPRINTED CHEMICAL DETECTION DEVICE AND METHOD

• US 20080268550A1
• Filed: 07/08/2003
• Published: 10/30/2008
• Est. Priority Date: 02/24/2003
• Status: Active Grant

• Current Assignee: National Research Council Canada
• Original Assignee: National Research Council Canada
• Inventors: Bensebaa, Farid, Py, Christophe, Faid, Karim
• Granted Patent: US 7,459,316 B2
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

A novel method of molecular imprinting is described. Using a modified soft lithography technique, a molecularly-imprinted chemical detection device comprising at least one molecularly-imprinted polymer capable of detecting at least one chemical target is produced. The device can be used in the field for in situ detection and quantification of chemical targets using standard surface analytical techniques.

Claims

1. A method of producing a molecularly-imprinted chemical detection device comprising the steps of

a) modifying the surface of a solid support through the attachment of functional groups;

b) reacting the solid support with a derivatized molecular target to form a molecular target bound solid support;

c) reacting the molecular target bound solid support with at least one guest molecule thus forming a solid support stamp having a binding cavity around the molecular target;

d) selecting an appropriate solid substrate for facilitating attachment of the binding cavity on the solid support stamp to the solid substrate;

e) applying the solid support stamp to a surface of the solid substrate to attach the binding cavity to the solid substrate; and

f) removing the molecular target and solid support to produce a molecularly-imprinted polymer on the surface of the solid substrate.

2. The method of claim 1 wherein the at least one guest molecule is at least one derivatized functional monomer forming at least one functional monomer recognition element around the molecular target.

3. The method of claim 2 wherein at least two functional monomer recognition elements are polymerized the form the binding cavity around the molecular target.

4. The method of claim 1 wherein the guest molecule is at least one derivatized functional polymer.

5. The method of claim 1 wherein a silicon wafer solid support is modified to facilitate the attachment in step (a).

6. The method of claim 1 wherein the surface of the solid support is modified with amino-siloxane.

30. The method of claim 1 wherein the solid substrate is a coated slide.

31. The method of claim 30 wherein the coated slide is a coated glass slide.

32. The method of claim 30 wherein the coated slide is coated with one of gold and silicon dioxide.

33. The method of claim 1 wherein a plurality of binding cavities are attached to the solid substrate producing a plurality of molecularly-imprinted polymers.

34. The method of claim 33 wherein at least two different binding cavities are attached to the solid substrate producing at least two different molecularly-imprinted polymers segregated into separate areas.

35. The method of claim 1 wherein the solid support stamp is applied to a surface of a solid substrate having an area of about 1 to 2 cm².

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36. A method of producing a solid support stamp comprising the steps of:

a) modifying the surface of a silicon wafer by attaching functional groups;

b) reacting the silicon wafer with a derivatized molecular target to form a molecular target bound solid support; and

c) reacting the molecular target bound solid support with at least one guest molecule thus forming a binding cavity around the molecular target.

37. The method of claim 36 wherein the at least one guest molecule is at least one derivatized functional monomer forming at least one functional monomer recognition element around the molecular target.

38. The method of claim 37 wherein at least two functional monomer recognition elements are polymerized to form the binding cavity around the molecular target.

39. The method of claim 36 wherein the guest molecule is at least one derivatized functional polymer.

40. A method of producing a molecularly-imprinted chemical detection device using a solid support stamp produced from a molecular target bound solid support, having a binding cavity around the molecular target comprising the steps of

a) selecting an appropriate solid substrate for facilitating attachment of the binding cavity on the solid support stamp to the solid substrate;

b) applying the solid support stamp to the surface of the solid substrate to attach the binding cavity to the solid substrate; and

c) removing the molecular target bound solid support to produce a molecularly-imprinted polymer on the surface of the solid substrate.

41. The method of claim 40 wherein the solid support stamp is applied to a surface of a coated slide in step (b).

42. The method of claim 41 wherein the coated slide is a coated glass slide.

43. The method of claim 41 wherein the coated slide is coated in one of gold and silicon dioxide.

44. The method of claim 40 wherein a plurality of binding cavities are attached to the solid substrate producing a plurality of molecularly-imprinted polymers.

45. The method of claim 40 wherein at least two different binding cavities are attached to the solid substrate producing at least two different molecularly-imprinted polymers segregated into separate areas.