Method of Performing a Microarray Assay

• US 20080269069A1
• Filed: 12/11/2006
• Published: 10/30/2008
• Est. Priority Date: 12/21/2005
• Status: Abandoned Application

• Current Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Original Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Inventors: Luedke, Gerd, Boos, Andreas, Bacher, Johannes
• Application Number:
  US12/158,074
• Publication Number:
  US 20080269069A1
• Time in Patent Office: Days
• US Class Current: 506/12
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2565/102 Multiple non-interacting labels
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/544 the carrier being organic

Abstract

Disclosed is a method for performing a microarray assay on one or more sample fluid(s), said fluids comprising target biological compounds. The method comprises the step of tagging said target biological compounds with labels. The following step comprises contacting said sample fluid(s) with a substrate and detecting the presence of said labels at the surface of said substrate. The method is suitable for the simultaneous analysis, in one microarray, of one or more types of target biological compounds, in one or more sample fluid(s). To this end each of said types of biological compounds is tagged with a different label so that target biological compounds belonging to different sample fluids have different labels. Said different labels are discriminable upon detection at the surface of said substrate. Also disclosed is the use of a polymer substrate in a method for performing a microarray assay.

Claims

1. A method for performing a microarray assay on one or more sample fluid(s) comprising target biological compounds, said method comprising tagging said target biological compounds with labels, contacting said sample fluid(s) with a substrate and detecting the presence of said labels at the surface of said substrate, wherein said method is suitable for the simultaneous analysis, in one microarray, of one or more types of target biological compounds, in one or more sample fluid(s), and wherein:

(i) each of said types of biological compounds is tagged with a different label so that target biological compounds belonging to different sample fluids have different labels,

(ii) at least one of the number of types of target biological compounds and the number of sample fluids is at least 2, and

(iii) said different labels are discriminable upon detection at the surface of said substrate.

2. A method according to claim 1, wherein said sample fluid(s) is (are) passed through said substrate.

3. A method according to claim 1, wherein said substrate is a porous substrate.

4. A method according to claim 1, wherein said substrate comprises a polymer.

5. A method according to claim 1, wherein comprises a polyamide homopolymer or copolymer.

6. A method according to claim 5, wherein said polyamide homopolymer or copolymer is modified by introduction of quaternary ammonium, solvolysis, or derivatization of amide groups into amidine groups

7. A method according to claim 1, wherein said substrate comprises a cellulosic material.

8. A method according to claim 1, wherein said substrate comprises a thermoplastic fluorinated polymer.

10. A method according to 1, wherein said different labels are selected from the group consisting of magnetic beads, radioactive isotopes, enzymes, calorimetric molecules and micro-bubbles.

9. A method according to wherein said different labels include luminescent molecules.

11. The use of a polymer substrate in a method for performing a microarray assay comprising contacting one or more sample fluid with a substrate, wherein said method permits the simultaneous analysis, in one microarray, of one or more types of target biological compounds, in one or more sample fluids wherein at least one of the number of types of target biological compounds and the number of sample fluids is at least 2.