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Autologous T Cell Manufacturing Processes

  • US 20080274091A1
  • Filed: 05/22/2006
  • Published: 11/06/2008
  • Est. Priority Date: 05/20/2005
  • Status: Abandoned Application
First Claim
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1. A method for stable transduction of primary cells of the hematopoietic system and/or hematopoietic stem cells, comprising contacting, in vitro or ex vivo, the surface of the cells with both a lentiviral vector and at least one molecule which binds the cell surface, and culturing the cells in a ventilated vessel comprising two or more layers under conditions conducive to growth and/or proliferation, wherein the vessel is suitable for culturing at least about 100 million cells.

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