Method for increasing the speed of nucleic acid amplification reactions
First Claim
1. A method of performing a nucleic acid amplification reaction where a reaction mixture is subjected sequentially to a selected denaturation temperature which is provided by a heat source and to a selected annealing, and/or extension temperature, said method comprising the step of moving said reaction mixture out of the influence of said heat source once said temperature is higher than said desired denaturation temperature and is lower than said desired annealing, and/or extension temperature.
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Abstract
The speed of a nucleic acid amplification reaction, such as the Polymerase Chain Reaction (PCR), can be increased by setting the temperature of heat sources above and below the desired denaturation, annealing, and extension temperatures. The reaction mixture is only contacted with the heat sources long enough for the desired temperatures to be reached.
71 Citations
28 Claims
- 1. A method of performing a nucleic acid amplification reaction where a reaction mixture is subjected sequentially to a selected denaturation temperature which is provided by a heat source and to a selected annealing, and/or extension temperature, said method comprising the step of moving said reaction mixture out of the influence of said heat source once said temperature is higher than said desired denaturation temperature and is lower than said desired annealing, and/or extension temperature.
Specification