BIOSENSORS FOR MEASURING ANALYTES IN THE INTERSTITIAL FLUID
First Claim
1. A method for determining the blood levels of glucose in a subject, the method comprisinga) inserting in the skin of the subject to a depth of up to about 2 mm from the surface of the skin, a sensor that produces a detectable signal in the presence of glucose in the interstitial fluid of the subject,b) maintaining said sensor in the skin at said depth such that at least a portion of said sensor is exposed to the interstitial fluid in the skin,c) measuring the detectable signal of the sensor produced by said sensor upon exposure to said interstitial fluid, andd) correlating the signal produced by the sensor to the level of blood glucose, thereby determining the levels of blood glucose in the subject.
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Abstract
The invention relates to methods and devices for measuring blood glucose levels in a subject, where the methods and devices are designed for exposing a sensing mechanism to interstitial fluid in the subject.
26 Citations
16 Claims
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1. A method for determining the blood levels of glucose in a subject, the method comprising
a) inserting in the skin of the subject to a depth of up to about 2 mm from the surface of the skin, a sensor that produces a detectable signal in the presence of glucose in the interstitial fluid of the subject, b) maintaining said sensor in the skin at said depth such that at least a portion of said sensor is exposed to the interstitial fluid in the skin, c) measuring the detectable signal of the sensor produced by said sensor upon exposure to said interstitial fluid, and d) correlating the signal produced by the sensor to the level of blood glucose, thereby determining the levels of blood glucose in the subject.
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12. A biosensor for detecting blood glucose levels in a subject, the biosensor comprising
a) a sensing mechanism that produces an optical signal in the presence of glucose said sensing mechanism comprising a fluorescently labeled glucose-galactose binding protein (GGBP) entrapped within a hydrogel matrix, b) a shallow-depth housing that encases the sensing mechanism, wherein the shallow-depth housing comprises a hollow needle, wherein said hollow needle is a 31 gauge needle or smaller and penetrates the skin of a subject to a depth of up to about 2 mm from the surface of the skin such that said sensing mechanism is exposed to interstitial fluid, and c) an optical conduit, wherein said optical conduit is in optical connectivity with said sensing mechanism and the needle houses at least a portion of said conduit wherein the optical signal of the sensing mechanism generated from exposure to the interstitial fluid of the subject is correlated to blood glucose levels.
Specification