LAMINATE SHEET ARTICLES FOR TISSUE REGENERATION

• US 20080279833A1
• Filed: 05/10/2007
• Published: 11/13/2008
• Est. Priority Date: 05/10/2007
• Status: Abandoned Application

• Current Assignee: Cormatrix Cardiovascular, Inc.
• Original Assignee: Cormatrix Cardiovascular, Inc.
• Inventors: Matheny, Robert G.
• Application Number:
  US11/747,004
• Publication Number:
  US 20080279833A1
• Time in Patent Office: Days
• US Class Current: 424/93.71
• CPC Class Codes: A61F 2/02 Prostheses implantable into the body closure means for urethra or rectum or for artificial body openings therefor A61F2/0004
• CPC Class Codes: A61F 2210/0076 multilayered, e.g. laminated structures
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/22 Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 35/38 Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
• CPC Class Codes: A61K 38/005 Enzyme inhibitors protease inhibitors A61K38/55
• CPC Class Codes: A61K 38/18 Growth factors; Growth regulators
• CPC Class Codes: A61K 38/1825 Fibroblast growth factor [FGF]
• CPC Class Codes: A61L 27/3629 Intestinal tissue, e.g. small intestinal submucosa
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3641 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3679 Hollow organs, e.g. bladder, esophagus, urether, uterus, intestine
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 31/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: C07K 14/435 from animals; from humans
• CPC Class Codes: C12N 5/068 Stem cells; Progenitors

Abstract

The invention is to articles of extracellular matrix. The articles comprise one or more sheets of mammalian extracellular matrix laminated together. A single sheet can be folded over and laminated on 3 sides. Two or more sheets can be laminated to each other at their edges. The sheets can further encase a composition comprising a cell or cells, such as for example, a stem cell. A single sheet can be folded over to encase a composition, or rolled to encase a composition with lamination at either end of the roll, for example. The invention also includes methods of using these articles to regenerate tissue at tissue defects, or heal wounds in damaged tissue.

Claims

1. An article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix.

2. The article of claim 1, wherein said planar laminated article comprises two sheets of mammalian extracellular matrix.

3. The article of claim 1, wherein said two or more sheets of mammalian extracellular matrix are from different mammalian tissue sources.

4. The article of claim 3, wherein a first sheet is from small Intestine submucosa (SIS) of a mammal, and a second sheet is from stomach submucosa (SS) of a mammal.

5. The article of claim 1, wherein said lamination is complete.

6. The article of claim 1, wherein said lamination is partial.

7. The article of claim 1, wherein said sheets are rectangular, and said planar laminated article is rectangular.

8. The article of claim 1, wherein said sheets are triangular, and said planar laminated article is triangular.

9. The article of claim 1, wherein said sheets are circular, and said planar laminated article is circular.

10. An article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix, said article further comprising in between at least two of said sheets at least one cell to further effect wound healing or tissue regeneration upon placement of said laminated article in a mammal at a site in said mammal in need of wound healing or tissue regeneration.

11. The article of claim 10, wherein said cell is a stem cell.

12. The article of claim 10, wherein said cell is selected from the group consisting of human embryonic stem cell, a fetal cardiomyocyte, a myofibroblast, a mesenchymal stem cell, an autotransplanted expanded cardiomyocyte, an adipocyte, a totipotent cell, a pluripotent cell, a blood stem cell, a myoblast, an adult stem cell, a bone marrow cell, a mesenchymal cell, an embryonic stem cell, a parenchymal cell, an epithelial cell, an endothelial cell, a mesothelial cell, a fibroblast, a myofibroblast, an osteoblast, a chondrocyte, an exogenous cell, an endogenous cell, a stem cell, a hematopoetic stem cell, a pluripotent stem cell, a bone marrow-derived progenitor cell, a progenitor cell, a myocardial cell, a skeletal cell, a fetal cell, an embryonic cell, an undifferentiated cell, a multi-potent progenitor cell, a unipotent progenitor cell, a monocyte, a cardiomyocyte, a cardiac myoblast, a skeletal myoblast, a macrophage, a capillary endothelial cell, a xenogenic cell, an allogenic cell, an adult stem cell, and a post-natal stem cell.

13. The article of claim 10, wherein said cell is a mesechymal cell.

14. A method comprising:

a. identifying a defect or wound in mammalian tissue which could benefit from tissue regeneration or wound healing,

b. providing an article comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix,

c. contacting said defect or wound with said article, and

d. regenerating tissue at said defect or healing said wound thereby.

15. The method of claim 14, wherein said defect or wound is in myocardial tissue.

16. The method of claim 14, wherein said defect or wound is in pancreatic tissue.

17. The method of claim 14, wherein said defect or wound is in liver tissue.

18. The method of claim 14, wherein said article further comprises a composition comprising a cell between at least two sheets of extracellular matrix.

19. The method of claim 18, wherein said cell is a stem cell.

20. The method of claim 18, wherein said cell is selected from the group consisting of human embryonic stem cell, a fetal cardiomyocyte, a myofibroblast, a mesenchymal stem cell, an autotransplanted expanded cardiomyocyte, an adipocyte, a totipotent cell, a pluripotent cell, a blood stem cell, a myoblast, an adult stem cell, a bone marrow cell, a mesenchymal cell, an embryonic stem cell, a parenchymal cell, an epithelial cell, an endothelial cell, a mesothelial cell, a fibroblast, a myofibroblast, an osteoblast, a chondrocyte, an exogenous cell, an endogenous cell, a stem cell, a hematopoetic stem cell, a pluripotent stem cell, a bone marrow-derived progenitor cell, a progenitor cell, a myocardial cell, a skeletal cell, a fetal cell, an embryonic cell, an undifferentiated cell, a multi-potent progenitor cell, a unipotent progenitor cell, a monocyte, a cardiomyocyte, a cardiac myoblast, a skeletal myoblast, a macrophage, a capillary endothelial cell, a xenogenic cell, an allogenic cell, an adult stem cell, and a post-natal stem cell.