HPV antigens, vaccine compositions, and related methods
First Claim
Patent Images
1. An isolated antigen comprising a component of human papilloma virus (HPV) fused to a thermostable protein;
- wherein the HPV component comprises at least one domain selected from the group consisting of E7 from HPV16, E7 from HPV18, E6 from HPV16, and E6 from HPV18, a fragment of E7 from HPV16, a fragment of E7 from HPV18, a fragment of E6 from HPV16, and a fragment of E6 from HPV18.
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Abstract
The present invention relates to the intersection of the fields of immunology and protein engineering, and particularly to antigens and vaccines useful in prevention of infection by human papilloma virus. Provided are recombinant protein antigens, compositions, and methods for the production and use of such antigens and vaccine compositions.
117 Citations
70 Claims
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1. An isolated antigen comprising a component of human papilloma virus (HPV) fused to a thermostable protein;
wherein the HPV component comprises at least one domain selected from the group consisting of E7 from HPV16, E7 from HPV18, E6 from HPV16, and E6 from HPV18, a fragment of E7 from HPV16, a fragment of E7 from HPV18, a fragment of E6 from HPV16, and a fragment of E6 from HPV18.
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2-10. -10. (canceled)
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11. A vaccine composition comprising an antigen comprising a component of human papilloma virus (HPV) fused to a thermostable protein and a pharmaceutically acceptable carrier;
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wherein the HPV component comprises at least one domain selected from the group consisting of E7 from HPV16, E7 from HPV18, E6 from HPV16, and E6 from HPV18, a fragment of E7 from HPV16, a fragment of E7 from HPV18, a fragment of E6 from HPV16, and a fragment of E6 from HPV18; and wherein the composition is capable of eliciting an immune response upon administration to a subject.
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21. (canceled)
- 28. A vaccine composition comprising at least two antigens, each of which comprises a component of human papilloma virus (HPV), wherein at least one of the antigens further comprises a thermostable protein, wherein the composition is capable of eliciting an immune response upon administration to an subject.
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30. A method for inducing a protective immune response against human papilloma virus (HPV) infection in a subject comprising administering to a subject an effective amount of an anti-HPV vaccine composition, wherein the administration is sufficient to stimulate production of antigen specific antibodies or stimulate a cellular immune response by the subject;
- thereby inducing a protective immune response;
a. wherein the vaccine composition comprises antigen comprising a component of human papilloma virus (HPV) fused to a thermostable protein; and b. wherein the HPV component comprises at least one domain at least one domain selected from the group consisting of E7 from HPV16, E7 from HPV18, E6 from HPV16, and E6 from HPV18, a fragment of E7 from HPV16, a fragment of E7 from HPV18, a fragment of E6 from HPV16, and a fragment of E6 from HPV18.
- thereby inducing a protective immune response;
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31-33. -33. (canceled)
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34. A method for producing an antigen protein comprising a component of human papilloma virus (HPV) fused to a thermostable protein, comprising:
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a. preparing a nucleic acid construct encoding an antigen component of human papilloma virus (HPV) fused to a thermostable protein; b. transforming a cell with the nucleic acid construct of step a; and c. incubating the cell under conditions favorable for expression of the antigen protein; d. thereby producing the antigen protein; e. wherein the HPV component of the antigen comprises at least one domain selected from the group consisting of selected from the group consisting of E7 from HPV16, E7 from HPV18, E6 from HPV16, and E6 from HPV18, a fragment of E7 from HPV16, a fragment of E7 from HPV18, a fragment of E6 from HPV16, and a fragment of E6 from HPV18.
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35-51. -51. (canceled)
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52. An isolated nucleic acid construct comprising nucleic acid sequence encoding a component of human papilloma virus (HPV) fused to a thermostable protein;
- wherein the HPV component comprises at least one domain selected from the group consisting of E7 from HPV16, E7 from HPV18, E6 from HPV16, and E6 from HPV18, a fragment of E7 from HPV16, a fragment of E7 from HPV18, a fragment of E6 from HPV16, and a fragment of E6 from HPV18 and combinations thereof.
- View Dependent Claims (66, 69)
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53-65. -65. (canceled)
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67-68. -68. (canceled)
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70-71. -71. (canceled)
Specification