Hybrid nucleic acid assembly

• US 20080280353A1
• Filed: 09/21/2007
• Published: 11/13/2008
• Est. Priority Date: 02/18/2000
• Status: Abandoned Application

• Current Assignee: Biomed Solutions, LLC
• Original Assignee: Biomed Solutions, LLC
• Inventors: Connelly, Patrick R., Weiner, Michael L., Custer, Andrew W.
• Application Number:
  US11/902,503
• Publication Number:
  US 20080280353A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2523/301 Sonication
• CPC Class Codes: C12Q 2563/137 Metal/ion, e.g. metal label
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence

Abstract

A detection device comprised of a hybrid nucleic acid assembly. The device further comprises a nucleic acid polymer, a first nanoparticle conjugated to the nucleic acid polymer, a second nanoparticle conjugated with the nucleic acid polymer, a means for introducing energy into the first nanoparticle, means for detecting energy from the second nanoparticle, and means for determining a physical property of the nucleic acid polymer, wherein the means of introducing energy into the first nanoparticle is a source of mechanical energy.

Claims

1-63. -63. (canceled)

64. A system for determining a physical property of a nucleic acid polymer or an amino acid polymer, comprising:

a polymer selected from the group consisting of a nucleic acid polymer and an amino acid polymer;

a first transceiver for receiving a first signal from an energy source and introducing said first signal to the polymer, wherein the first transceiver is conjugated to the polymer;

a second transceiver for receiving a second signal from the polymer, wherein the second transceiver is conjugated to the polymer;

a device for producing a third signal containing information regarding a difference between the first and second signals.

65. The system according to claim 64, wherein the signal provided by the energy source is selected from the group consisting of mechanical, ultrasonic, light, laser, magnetic and radio wave energy.

66. The system according to claim 64, wherein each of the first and second transceivers is independently selected from a nanotube, a nanoparticle, a fluorophore or a dendrimer.

67. The system according to claim 64, wherein the polymer is an amino acid polymer, and the amino acid polymer is a protein or a peptide.

68. The system according to claim 64, wherein at least one of the first and second transceivers comprises gold.

69. The system according to claim 64, wherein at least one of the first and second transceivers is a carbon nanotube.

70. The system according to claim 64, wherein the polymer is a nucleic acid polymer which is a single-strand polymer or a double-strand polymer.

71. The system according to claim 64, wherein the first and second transceivers and the signal-producing device are transfected into a cell.

72. The system according to claim 71, wherein said cell is located in a cell culture apparatus.

73. The system according to claim 64, wherein the assembly is located within an organism comprised of one or more cells.

74. The system according to claim 64, wherein the polymer is an amino acid polymer comprised of a proximal end and a distal end, and wherein a first antibody is bound to a first antigenic site of the amino acid polymer, and a second antibody is bound to a second antigenic site on the amino acid polymer.

75. The system according to claim 64, wherein the produced signal is a radio frequency signal.

76. The system according to claim 64, further comprising a device for receiving the third signal and determining a physical property of the nucleic acid polymer or amino acid polymer.

77. The system according to claim 64, wherein at least one of the first and second transceivers is a dendrimer consisting essentially of a conductive polymer.

78. The system according to claim 77, wherein the dendrimer has a shape selected from the group consisting of a rod shape form, a spiral shape form, a star shape form, a starburst shape form and a spherical shape form.

79. The system according to claim 64, wherein the energy detection device is selected from the group consisting of a microvoltage potential detector, a microcurrent detector, an ultrasonic transducer, a microprocessor control unit, a nanoparticle sensor, a fluorophore, a chromophore, a nanotube or a quantum dot.