LIGHT SCATTERING DETECTOR

• US 20080285032A1
• Filed: 08/03/2007
• Published: 11/20/2008
• Est. Priority Date: 08/04/2006
• Status: Abandoned Application

• Current Assignee: Shimadzu Corporation
• Original Assignee: Shimadzu Corporation
• Inventors: Ohkubo, Kunihiko
• Application Number:
  US11/833,659
• Publication Number:
  US 20080285032A1
• Time in Patent Office: Days
• US Class Current: 356/343
• CPC Class Codes: G01N 15/0205 by optical means
• CPC Class Codes: G01N 2015/0092 Monitoring flocculation or agglomeration

Abstract

The light scattering detector according to the present invention aims at simultaneously measuring the molecular weight and size of particles having varieties of diameters. This detector facilitates the measurement operation and knowing how particles associate or dissociate as time progresses. In this detector, the emitted light (static light scattering measurement light) having a first wavelength by the first light source and the emitted light (dynamic light scattering measurement light) having a second wavelength which is different from the first wavelength by the second light source are combined by a beam splitter 5 and coaxially directed onto the sample sell 10 to which a liquid sample S is supplied. While the irradiated area by the static light scattering measurement light is large, the irradiated area by the dynamic light scattering measurement light, which is coherent light, is narrow. Detectors 12 which selectively detect the first wavelength and detectors 13 which selectively detect the second wavelength are placed so as to encircle the sample cell 10. The detection signals by the detectors 11, 12 and the detection signals by the detectors 13 are separately mathematically-operated by a data processor 15 to calculate the molecule weight and size of the particles in the sample S.

Claims

1. A light scattering detector for detecting a microscopic particle in a liquid or gaseous sample, the detector comprising:

a) a transparent sample cell for holding a sample;

b) a first light source for emitting a static light scattering measurement light having a first wavelength or a first range of wavelengths;

c) a second light source for emitting a dynamic light scattering measurement light having a second wavelength or a second range of wavelengths which are different from the first wavelength or the first range of wavelengths;

d) a first detecting unit comprising plural detectors arranged so as to encircle the sample cell, the detector being capable of selectively detecting the first wavelength or the first range of wavelengths in order to detect lights scattered at different scattering angles around the sample cell in response to an irradiation of the static light scattering measurement light;

e) a second detecting unit comprising one or plural detectors which are arranged so as to encircle the sample cell, the detector being capable of selectively detecting the second wavelength or the second range of wavelengths in order to detect lights scattered at different scattering angles around the sample cell in response to an irradiation of the dynamic light scattering measurement light; and

f) a mathematical processor for receiving a detection signal from the first detecting unit and a detection signal from the second detecting unit in parallel when both the static light scattering measurement light by the first light source and the dynamic light scattering measurement light by the second light source are irradiated onto the sample cell at a same time, and then performing both a mathematical operation by a static light scattering method based on the former detection signal and a mathematical operation by a dynamic light scattering method based on the latter detection signal.

2. The light scattering detector of claim 1, wherein both of the incident lights by the first light source and the second light source are coherent.

3. The light scattering detector of claim 1, wherein the incident light by the first light source is incoherent and the incident light by the second light source is coherent.

4. The light scattering detector of claim 1, wherein a light flux of the incident light by the first light source is larger than a beam size of the incident light by the second light source.

5. The light scattering detector of claim 1, further comprising a light combiner for introducing the incident light by the first light source and the incident light by the second light source to the sample cell along a same light path.

6. The light scattering detector of claim 1, further comprising a transmitted light detector for detecting a light which has passed through the sample cell in response to the incident light by the first light source.

7. A light scattering detector for detecting a microscopic particle in a liquid or gaseous sample, the detector comprising:

a) a transparent sample cell for holding a sample;

b) a first light source for emitting a static light scattering measurement light;

c) a second light source for emitting a dynamic light scattering measurement light;

d) an incident light switcher for time-divisionally irradiating the sample cell with the static light scattering measurement light by the first light source and the dynamic light scattering measurement light by the second light source;

e) a detecting unit comprising plural detectors which are arranged so as to encircle the sample cell and detect lights scattered at different scattering angles around the sample cell in response to an irradiation of the static light scattering measurement light or the dynamic light scattering measurement light; and

f) a mathematical processor for separating a detection signal from the detecting unit into a static light scattering detection signal and a dynamic light scattering detection signal in synchronization with a time-divisionally switching of incident lights by the incident light switcher, and then performing both a mathematical operation by static light scattering method based on the former detection signal and a mathematical operation by dynamic light scattering method based on the latter detection signal.

8. The light scattering detector of claim 7, wherein both of the incident lights by the first light source and the second light source are coherent.

9. The light scattering detector of claim 7, wherein the incident light by the first light source is incoherent and the incident light by the second light source is coherent.

10. The light scattering detector of claim 7, wherein a light flux of the incident light by the first light source is larger than a beam size of the incident light by the second light source.

11. The light scattering detector of claim 7, wherein the incident light switcher selectively introduces either the incident light by the first light source or the incident light by the second light source to the sample cell along a same light path.

12. The light scattering detector of claim 7, further comprising a transmitted light detector for detecting a light which has passed through the sample cell in response to the incident light by the first light source.