Systems and methods for detecting toxins in a sample

• US 20080286822A1
• Filed: 06/29/2007
• Published: 11/20/2008
• Est. Priority Date: 05/18/2007
• Status: Abandoned Application

• Current Assignee: Woods Hole Oceanographic Institution
• Original Assignee: Woods Hole Oceanographic Institution
• Inventors: Gallager, Scott
• Application Number:
  US11/824,138
• Publication Number:
  US 20080286822A1
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: G01N 33/1866 using microorganisms

Abstract

In one aspect the invention provides a system for detecting the presence of toxins in a sample that includes a plurality of chambers for culturing organisms and observing the organism'"'"'s motility response when introduced into a sample containing a toxin. The toxicity measurement system may include an imaging module to monitor and track the movement of one or more organisms in the sample and identify abnormalities. In other aspects, the invention provides methods of culturing organisms and detecting the presence of toxins in the sample using the motility response of organisms in the sample.

Claims

1. An apparatus to identify the presence of a toxin in a sample, comprising

a first chamber containing an organism having a motility response to a toxin in the sample,

a second chamber, having a fluid connection with the first chamber, and having an opening such that sample may be introduced through the opening and combined with the organism from the first chamber,

an imaging module, electromagnetically coupled to the second chamber, capable of imaging a path of the organism in the second chamber, and

a processing module connected to the imaging module such that the imaged path is processed to detect the presence of a toxin in the sample.

2. An apparatus of claim 1, further comprising a sampling pump and valve for regulating the movement of the organism and sample into the second chamber.

3. An apparatus of claim 2, wherein the sampling pump and valve includes a syringe type pump and valve system.

4. An apparatus of claim 2, further comprising at least one supply unit in fluid connection with the sampling pump and valve for supplying at least one of acid, bleach, water and the sample.

5. An apparatus of claim 1, wherein the organism includes at least one of a single celled organism, multi-celled organism, fresh water organism and salt water organism.

6. An apparatus of claim 1, wherein the organism includes at least one of a ciliate, flagellate and marine algae.

7. An apparatus of claim 6, wherein the ciliate includes at least one of Tetrahymena pyriformis, Tetrahymena malaccensus, Tetrahymena furgisoni, and Glaucoma.

8. An apparatus of claim 6, wherein the flagellate includes at least one of Tetramitus and Bodo.

9. An apparatus of claim 6, wherein the marine algae includes at least one of Chlorophycia, rhodomonas, heterocapsa, dunaliella, and chlamydomonas.

10. An apparatus of claim 1, wherein the sample includes water obtained from at least one of public water supply, reservoirs, taps, treatment plants, military portable water tanks and field installations.

11. An apparatus of claim 1, wherein the sample includes a fluid.

12. An apparatus of claim 1, wherein the toxin includes at least one of heavy metals, cellular respiration inhibitors, cellular energy transfer disruptors, carcinogens, acetylcholinesterase inhibitors, reactive oxygen species, general oxidants, substances influencing sugar metabolism, and water disinfection products.

13. An apparatus of claim 1, wherein the toxin includes at least one of cadmium chloride, potassium cyanide, sodium azide, ethylene glycol, sodium arsenate, Alflatoxin B, parathion, paraquat, methane methyl sulfonate (MMS), hydrogen peroxide, methyl nitrosoamine, and chlorine.

14. An apparatus of claim 1, wherein the first chamber includes at least one of small-volume tubes, IV bags, spinner flasks and plastic bags.

15. An apparatus of claim 1, wherein the second chamber includes a transparent glass enclosure.

16. A method of detecting the presence of a toxin in a sample, comprising the steps of

culturing an organism, having a motility response to a toxin, in a first medium,

culturing the organism in a second medium such that the second medium is removed and replenished at regular intervals of time,

introducing the cultured organism to a sample at regular intervals of time, and

detecting the presence of the toxin in the sample based at least on a motility response of the cultured organism to the toxin.

17. A method of claim 16, wherein the first medium includes at least one of a bacteria-based medium and a yeast based medium.

18. A method of claim 16, wherein the step of culturing the organism in the first medium includes performing a static culture.

19. A method of claim 16, wherein the step of culturing the organism in the second medium includes performing a continuous culture.

20. A method of claim 16, wherein the first medium includes at least one of MS1, yeast extract.

21. A method of claim 16, wherein the second medium includes at least one of MS1, yeast extract.