Self-Processing Plants and Plant Parts
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Abstract
The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.
35 Citations
265 Claims
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1-234. -234. (canceled)
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235. An isolated polynucleotide a) comprising SEQ ID NO:
- 61, 63, 65, 69, 73, 74, 75, 76, 77, 78, 79, 81, 83, 85, 87, 89, 91, 93, 94, 95, 96, 97, 99, 101, 103, 105, 107, 109, or 111, or the complement thereof, or a polynucleotide which hybridizes to the complement of any one of SEQ ID NO;
61, 63, 65, 73, 74, 75, 76, 77, 78, 79, 81, 83, 85, 87, 89, 91, 93, 94, 95, 96, 97, 99, 101, 103, 105, 107, 109, or 111, under low stringency hybridization conditions and encodes a polypeptide having cellulase, hemicellulase, exo-1,4-β
-cellobiohydrolase, exo-1,3-β
-D-glucanase, β
-glucosidase, endoglucanase, L-arabinase, α
-arabinosidase, galactanase, galactosidase, mannanase, mannosidase, xylanase, xylosidase, protease, glucanase, or esterase activity or b) encoding a polypeptide comprising SEQ ID NO;
62, 64, 66, 70, 80, 82, 84, 86, 88, 90, 92, 100, 102, 104, 106, 108, 110, or 112, or an enzymatically active fragment thereof. - View Dependent Claims (236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254)
- 61, 63, 65, 69, 73, 74, 75, 76, 77, 78, 79, 81, 83, 85, 87, 89, 91, 93, 94, 95, 96, 97, 99, 101, 103, 105, 107, 109, or 111, or the complement thereof, or a polynucleotide which hybridizes to the complement of any one of SEQ ID NO;
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255. A method for preparing fermentable sugar, monosaccharide, or oligosaccharide comprising the steps of treating a transgenic monocot plant part comprising an enzyme cellulase, hemicellulase, exo-1,4-b-cellobiohydrolase, exo-1,3-b-D-glucanase, b-glucosidase, endoglucanase, L-arabinase, α
- -arabinosidase, galactanase, galactosidase, mannanase, mannosidase, xylanase, xylosidase, protease, glucanase, or esterase under conditions to activate the enzyme thereby digesting polysaccharide to form oligosaccharide, monosaccharide, or fermentable sugar, wherein the plant part is obtained from a transformed monocot plant, the genome of which is augmented with an expression cassette comprising a promoter operably linked to a polynucleotide encoding the enzyme and a targeting sequence.
- View Dependent Claims (256, 257, 258, 259, 260, 261, 262, 263, 264, 265)
Specification