Thermal Reaction Device and Method for Using the Same
1 Assignment
0 Petitions
Accused Products
Abstract
An M.times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
-
Citations
27 Claims
-
1-19. -19. (canceled)
-
20. A microfluidic device comprising:
-
a first layer and a second layer; a plurality of reaction cells, each reaction cell comprising a first chamber and a second chamber, said first chamber and said second chamber being in fluid communication through an interface channel having an interface valve associated therewith for controlling fluid communication between said first chamber and said second chamber wherein said chambers and said interface channel are present in the second layer; wherein each said first chamber is in fluid communication with a first fluid channel located in said second layer, and each said first fluid channel is in fluid communication with a plurality of first chambers; wherein each said second chamber is in fluid communication with a second fluid channel, said second fluid channel is in fluid communication with a third fluid channel that traverses an interface between said first and second layers; and
wherein a plurality of said third fluid channels are in fluid communication with one fourth fluid channel, and each said fourth fluid channel is located in said first layer. - View Dependent Claims (21, 22, 23, 24, 25, 26)
-
-
27. A microfluidic device comprising:
-
a first layer and a second layer; a plurality of reaction cells, each reaction cell comprising a first chamber and a second chamber, said first chamber and said second chamber being in fluid communication through an interface channel having an interface valve associated therewith for controlling fluid communication between said first chamber and said second chamber wherein said chambers and said interface channel are present in the second layer; wherein each said first chamber is in fluid communication with a first fluid channel located in said second layer, and each said first fluid channel is in fluid communication with a plurality of first chambers; wherein each said second chamber is in fluid communication with a fluid channel that traverses an interface between said first and second layers; and
wherein a plurality of said fluid channels traversing said interface are in fluid communication with a common fluid channel located in said first layer.
-
Specification