Hyaluronate synthase gene and uses thereof

• US 20080293108A1
• Filed: 02/11/2008
• Published: 11/27/2008
• Est. Priority Date: 07/01/1994
• Status: Abandoned Application

• Current Assignee: John Papaconstantinou, Paul H. Weigel, Paul L. Deangelis
• Original Assignee: John Papaconstantinou, Paul H. Weigel, Paul L. Deangelis
• Inventors: Papaconstantinou, John, DeAngelis, Paul L., Weigel, Paul H.
• Application Number:
  US12/069,513
• Publication Number:
  US 20080293108A1
• Time in Patent Office: Days
• US Class Current: 435/101
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12Y 204/01212 Hyaluronan synthase (2.4.1.212)

Abstract

Disclosed are DNA sequences encoding hyaluronic acid synthase that are employed to construct recombinant cells useful in the production of hyaluronata synthase and hyaluronic acid (HA). In preferred aspects, chromosomal DNA encoding the HA synthase gene, hasA, was cloned from a Streptococcus pyoganas genomic library. These vectors were used to transform host cells such as E. coli and acapsular Streptococci to produce hyaluronic acid. Resultant transformants were screened to identify colonies which have incorporated HA synthase DNA in a form that is being actively transcribed into the corresponding HA synthase enzyme. These colonies were selected and employed in the production of hyaluronic acid.

Claims

1. A purified nucleic acid segment encoding hyaluronan (HA) synthase.

2. A recombinant vector comprising the purified nucleic acid segment of claim 1.

3. A recombinant host cell comprising the recombinant vector of claim 2.

4. A method of producing HA, comprising the steps of:

providing the recombinant host cell of claim 3;

growing the recombinant host cell in a medium to secrete hyaluronic acid; and

recovering the secreted hyaluronic acid.