BIOLOGICALLY ACTIVE SUBSTANCE TRANSFER SHEET, CELL CULTURE KIT CONSTITUTED OF CELL CULTURE PLATE AND BIOLOGICALLY ACTIVE SUBSTANCE TRANSFER SHEET, PRODUCING METHOD THEREOF AND METHOD FOR SCREENING CELL CULTURE CONDITIONS UTILIZING THE SAME

• US 20080293138A1
• Filed: 07/30/2008
• Published: 11/27/2008
• Est. Priority Date: 12/16/2003
• Status: Active Grant

• Current Assignee: Brad Jackson
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Miyazaki, Takeshi, Watanabe, Kohei, Kanome, Osamu, Matsuda, Ryoichi, Fujiyama, Tomoyo
• Granted Patent: US 7,960,168 B2
• Time in Patent Office: Days
• US Class Current: 435/383
• CPC Class Codes: B01L 2300/0812 Bands; Tapes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0262 using touch-off at substrate or container
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025

Abstract

The invention provides a biologically active substance transfer sheet which can be formed with simple steps, a cell culture kit which comprises a cell culture plate and a biologically active substance transfer sheet, a producing method therefor, and a method for screening cell culture conditions with cells. The transfer sheet is prepared by providing biologically active substances having biological activity in plural areas on a sheet base, and biologically active substances are supplied from the sheet to culture regions provided on the plate.

Claims

1-10. -10. (canceled)

11. A cell culture kit comprising:

a culture plate having at least one culture region for culturing a cell; and

a sheet having a portion to cover the culture region;

wherein each of the culture region and the covering portion holds at least one substance having a biological activity to a cell, and at least one biologically active substance held by the culture region or the covering portion is immobilized thereon.

12. The cell culture kit according to claim 11, wherein the kit contains two or more combinations of the culture regions and the covering portions.

13. The cell culture kit according to claim 11, wherein the biologically active substance held in the culture region is immobilized thereto, the biologically active substance held in the covering portion is attached so as to be released in contact with a culture liquid, and the sheet base and the covering portion constitute a transfer sheet for transferring the biologically active substance to the culture region.

14. The cell culture kit according to claim 11, wherein two or more culture regions are present holding the biologically active substance in different concentrations.

15. The cell culture kit according to claim 11, wherein the culture region is formed in a recess formed in the plate.

16. The cell culture kit according to claim 11, wherein the culture region is surrounded by a protruding wall-shaped structure.

17. The cell culture kit according to claim 11, wherein a layer for holding a biologically active substance is formed on an entire or partial surface of the sheet base.

18. The cell culture kit according to claim 17, wherein the holding layer is able to release the biologically active substance in a sustainable manner or the layer releases a biologically active substance provided with a property for sustained release.

19. The cell culture kit according to claim 11, wherein the covering portion is formed in a recess formed in the sheet base.

20. The cell culture kit according to claim 11, wherein the covering portion is formed on a protruding portion formed on the sheet base.

21. The cell culture kit according to claim 11, wherein the covering portion is surrounded by a protruding wall-shaped structure formed on the sheet base.

37. A cell culture method using a cell culture kit as defined in any one of claims 11 to 21, comprising a step of placing the sheet on the culture plate to cover the culture region with the covering portion and a step of contacting a cell with a biologically active substance in a culture liquid in the culture region.

22-36. -36. (canceled)