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FLUORESCENT PROBES FOR DNA DETECTION BY HYBRIDIZATION WITH IMPROVED SENSITIVITY AND LOW BACKGROUND

  • US 20080293586A1
  • Filed: 03/12/2008
  • Published: 11/27/2008
  • Est. Priority Date: 10/28/2003
  • Status: Active Grant
First Claim
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1. An oligonucleotide-probe comprising an oligonucleotide portion having a 3′

  • -end and a 5′

    -end, a minor groove binder moiety attached to at least one of said nucleotide units through a linking group which covalently binds the minor groove binder moiety to the oligonucleotide, and a fluorophore and quencher, said probe having the formula;

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