Particle Fraction Determination of A Sample
First Claim
Patent Images
1. A method for determining a particle fraction of a fluid sample comprising the step of:
- measuring impedance or resistance of said fluid sample, wherein said fluid sample volume is up to 20 μ
L.
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Abstract
The present invention provides a device, test cards, methods and kits which are useful for determining the particle fraction and rate of viscosity of a fluid sample, the presence of an analyte in a fluid sample, or the aggregation of particles in a fluid sample to detect an analyte or as an immunologic assay.
175 Citations
56 Claims
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1. A method for determining a particle fraction of a fluid sample comprising the step of:
- measuring impedance or resistance of said fluid sample, wherein said fluid sample volume is up to 20 μ
L. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- measuring impedance or resistance of said fluid sample, wherein said fluid sample volume is up to 20 μ
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10. A method for determining particle fraction of a fluid sample comprising the step of:
- applying said fluid sample to a chamber having volume up to 2 μ
L; and
measuring an impedance or resistance of said fluid sample in said chamber. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
- applying said fluid sample to a chamber having volume up to 2 μ
- 19. A method for determining particle fraction of a fluid sample comprising the step of measuring electrical properties of said fluid sample, wherein said measuring step is used to determine said particle fraction, and wherein said fluid sample volume is up to 2 μ
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28. A method of determining particle fraction and viscosity of a fluid sample comprising the steps of:
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a) applying said fluid sample to a first chamber having volume up to 2 μ
L and a second chamber having volume up to 2 μ
L wherein the first chamber further comprises one or more reagents for accelerating a coagulation of a fluid sample upon contact with said fluid sample;b) measuring impedance of said fluid sample at one or more time points in the second chamber and correlating the impedance to a particle fraction calibration standard curve to produce a first impedance correlation; c) measuring a rate of change of measured impedance over time of said fluid sample in said first chamber and correlating said impedance to a rate of coagulation calibration standard curve to produce a second impedance correlation; and d) processing said first and second impedance correlations into output results corresponding to a particle fraction and viscosity of said fluid sample. - View Dependent Claims (29, 30, 31, 32)
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- 33. A method for determining the amount or presence of analyte in a fluid sample comprising measuring change of impedance or resistance of said fluid sample in a chamber wherein chamber comprises a binding moiety that selectively binds to said analyte and wherein volume of said chamber is up to 2 μ
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39. A method for determining the amount or presence of analyte in a fluid sample, wherein said determining step comprises:
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a) applying said fluid sample to two chambers, said chambers having volume up to 2 μ
L, wherein a first chamber comprises a binding moiety for agglutinating an analyte in a fluid sample upon contact with said fluid sample and a second chamber does not comprise said binding moiety;b) applying AC or DC electric potential to said first and second chambers; c) measuring the difference in impedance or resistance of said fluid sample in said first and second chambers at one or more intervals; d) correlating said difference in impedance or resistance to an analyte calibration standard curve to produce an impedance correlation; and e) processing said impedance or resistance correlation into output results corresponding to the amount or presence of said analyte of said fluid sample. - View Dependent Claims (40, 41, 42, 43)
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44. A device for determining particle fraction, amount or presence of an analyte, and viscosity of a fluid sample comprising;
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a) a detection unit adapted and configured to apply AC electric potential to said fluid sample and measure one or more impedance signals of said fluid sample; b) a processor unit electrically connected to said detection unit adapted and configured to receive and convert said impedance signals into output results corresponding to said particle fraction, amount or presence of said analyte, or viscosity of said fluid sample. - View Dependent Claims (45, 46, 47, 48, 49)
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50. A test card for determining a condition of a fluid sample comprising:
- an inlet port for receiving a fluid sample of less than 20 μ
L fluidly connected to two or more capillary channels wherein each capillary channel terminates in a fixed volume chamber having a volume up to 2 μ
L. - View Dependent Claims (51, 52, 53, 54, 55, 56)
- an inlet port for receiving a fluid sample of less than 20 μ
Specification