A-BETA IMMUNOGENIC PEPTIDE CARRIER CONJUGATES AND METHODS OF PRODUCING SAME
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Abstract
The present invention is directed to methods of producing conjugates of Aβ peptide immunogens with protein/polypeptide carrier molecules, which are useful as immunogens, wherein peptide immunogens are conjugated to protein carriers via activated functional groups on amino acid residues of the carrier or of the optionally attached linker molecule, and wherein any unconjugated reactive functional groups on amino acid residues are inactivated via capping, thus retaining the immunological functionality of the carrier molecule, but reducing the propensity for undesirable reactions that could render the conjugate less safe or effective. Furthermore, the invention also relates to such immunogenic products and immunogenic compositions containing such immunogenic products made by such methods.
93 Citations
397 Claims
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1-379. -379. (canceled)
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380. A method for inducing an immune response in a mammalian subject, the method comprising administering to the subject an effective amount of a immunogenic composition comprising an immunogenic conjugate of a peptide immunogen with a carrier protein, the immunogenic conjugate generated by:
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(a) introducing a reactive group into an amino acid residue of the peptide immunogen, wherein the peptide immunogen is an Aβ
peptide or a fragment or analog thereof;(b) derivatizing one or more functional groups of the carrier protein to generate an activated functional group on the carrier protein; (c) reacting the peptide immunogen of step (a) with the carrier protein of step (b) under conditions to form a conjugate, wherein the reactive group of the peptide immunogen is covalently attached to the activated functional group on the carrier protein; and (d) further reacting the conjugate of step (c) with a capping reagent to inactivate any remaining activated functional group on the carrier protein to generate the immunogenic conjugate. - View Dependent Claims (381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397)
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Specification