Bio Chip Device with a Sample Compartment and a Light Sensitive Element, Method for the Detection of Fluorescent Particles Within at Least One Sample Compartment of a Bio Chip Device

• US 20080300146A1
• Filed: 11/23/2006
• Published: 12/04/2008
• Est. Priority Date: 11/29/2005
• Status: Abandoned Application

• Current Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Original Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Inventors: Johnson, Mark Thomas, Van Herpen, Maarten Marinus Johannes Wilhelmus, Klunder, Derk Jan Wilfred, Ponjee, Marc Wilhelmus Gijsbert, Balistreri, Marcello Leonardo Mario
• Application Number:
  US12/095,145
• Publication Number:
  US 20080300146A1
• Time in Patent Office: Days
• US Class Current: 506/12
• CPC Class Codes: G01N 21/6454 using an integrated detector array

Abstract

The invention provides a bio chip device comprising at least one sample compartment and at least one light sensitive element, the at least one sample compartment being provided on a first side of the at least one light sensitive element, wherein incident light is provided incident from a second side opposite of the first side of the at least one light sensitive element. Further, the invention provides a method for the detection of fluorescent particles within at least one sample compartment of a bio chip device.

Claims

1. Bio chip device (10) comprising at least one sample compartment (40) and at least one light sensitive element (70), the at least one sample compartment (40) being provided on a first side (71) of the at least one light sensitive element (70), wherein incident light (20) is provided incident from a second side (72) opposite of the first side (71) of the at least one light sensitive element (70).

2. Bio chip device (10) according to claim 1, wherein the bio chip device (10) comprises a lid (50) on the first side (71) of the at least one light sensitive element (70), wherein the lid (50) is provided as an antireflective lid (50).

3. Bio chip device (10) according to claim 2, wherein the at least one sample compartment (40) is provided between the lid (50) and the at least one light sensitive element (70).

4. Bio chip device (10) according to claim 1, wherein the bio chip device (10) extends parallel to a detection plane (11), wherein the at least one light sensitive element (70) is provided in the detection plane (11), wherein at least one first filter element (25) is provided such that the incident light (20) is filtered prior to passing the detection plane (11) from the second side (72).

5. Bio chip device (10) according to claim 1, wherein the bio chip device (10) comprises at least one second filter means (60), wherein the at least one second filter means (60) is provided between the at least one sample compartment (40) and the at least one light sensitive element (70).

6. Bio chip device (10) according to claim 1, wherein the bio chip device (10) comprises a shielding means (75) provided on the second side (72) of the at least one light sensitive element (70), wherein the shielding means (75) prevents the incident light (20) from reaching directly the at least one light sensitive element (70).

7. Bio chip device (10) according to claim 1, wherein the bio chip device (10) extends parallel to a detection plane (11), wherein the at least one light sensitive element (70) is provided in the detection plane (11), wherein at least one deflection element (30) is provided in the detection plane (11) adjacent of the at least one light sensitive element (70).

8. Bio chip device (10) according to claim 7, wherein the at least one deflection element (30) comprises a forward scattering medium (31) .

9. Bio chip device (10) according to claim 7, wherein the at least one deflection element (30) comprises a lens (32).

10. Bio chip device (10) according to claim 7, wherein the at least one deflection element (30) comprises a deflection area (33) having a low refractive index compared to the forward scattering medium (31).

11. Bio chip device (10) according to claim 4, wherein the at least one first filter means (25) comprises at least one first polarisation filter (26) and wherein the at least one second filter means (60) comprises at least one second polarisation filter (61).

12. Bio chip device (10) according to claim 11, wherein the at least one first polarisation filter (26) is most permeable for linearly polarised light in a first polarisation plane and wherein the at least one second polarisation filter (61) is most permeable for linearly polarised light in a second polarisation plane.

13. Bio chip device (10) according to claim 11, wherein the at least one first polarisation filter (26) is most permeable for circularly polarised light in a first polarisation sense and wherein the at least one second polarisation filter (61) is most permeable for circularly polarised light in the first polarisation sense and wherein the bio chip device (10) comprises a lid (50) on the first side (71) of the at least one light sensitive element (70), wherein the lid (50) is provided as a reflective lid (50).

14. Bio chip device (10) according to claim 13, wherein the lid (50) is provided by a metal lid (50).

15. Bio chip device (10) according to claim 1, wherein the bio chip device (10) comprises a first substrate (15), wherein the first substrate (15) is provided as an optically transparent substrate (15), wherein the bio chip device (10) comprises a shielding means (75), wherein the bio chip device (10) further comprises at least one second filter means (60), wherein the shielding means (75), the at least one light sensitive element (70) and the second filter means (60) are provided at one side of the first substrate (15).

16. Bio chip device (10) according to claim 1, wherein the bio chip device (10) comprises a second substrate (16), wherein the at least one sample compartment (40) and the lid (50) are fixed by means of the second substrate (16).

17. Method for the detection of fluorescent particles (45) within at least one sample compartment (40), the method comprising the use of a bio chip device (10) comprising at least one light sensitive element (70), the at least one sample compartment (40) being provided on a first side (71) of the at least one light sensitive element (70), wherein a light source (21) produces incident light (20) from a second side (72) opposite of the first side (71) of the at least one light sensitive element (70).

18. Method according to claim 17, wherein incident light (20) is absorbed by a lid (50).

19. Method according to claim 17, wherein incident light (20) is linearly polarised in a first polarisation plane (101).

20. Method according to claim 17, wherein incident light (20) is circularly polarised in a first polarisation sense (103).

21. Method according to claim 20, wherein incident light (20) is reflected by a lid (50).