Methods and Apparatus For Measuring the Concentration of a Substance in a Solution
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Accused Products
Abstract
The present invention relates to methods and apparatus for detecting and measuring the concentration of a substance in a solution, the substance having an absorption at 300 nm or less. The methods and apparatus have particular utility in detecting and measuring the concentration of proteins and nucleic acids.
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Citations
39 Claims
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1-25. -25. (canceled)
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26. :
- A method for measuring the concentration of a substance in a solution, said substance having an absorption at 300 nm or less, said method comprising;
i) separating said substance from one or more compounds present in said solution by chromatography; ii) transmitting ultraviolet light of wavelength 300 nm or less from a light source through a band pass filter; iii) passing said ultraviolet light emanating from said band pass filter through a beam-splitter; iv) diverting a first portion of the light by the beam-splitter and quantifying the electromagnetic radiation of said first portion with a reference detector to obtain a reference value; v) irradiating a known path length of the solution with a second portion of the light passing through the beam splitter; vi) quantifying the electromagnetic radiation transmitted through the solution with a sample detector to obtain a sample value; and vii) calculating the absorbance (A) from said sample value and said reference value; wherein said light source is a light emitting diode which emits light of wavelength of 300 nm or less. - View Dependent Claims (27, 28, 29, 30, 31, 32)
- A method for measuring the concentration of a substance in a solution, said substance having an absorption at 300 nm or less, said method comprising;
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33. :
- An apparatus for measuring the concentration of a substance in a solution, said substance having an absorption at 300 nm or less, comprising;
i) a cell of known path length for containing said solution, said cell being transparent to light of wavelength of 300 nm or less; ii) a light source, wherein said light source is a light emitting diode which emits light of wavelength of 300 nm or less; iii) a band pass filter; iv) a beam splitter for dividing light into a first portion and a second portion; v) a reference detector for detecting electromagnetic radiation diverted by said beam splitter; vi) a sample detector for detecting the electro magnetic radiation transmitted through said solution; and vii) a chromatographic separation means for separating the substance from one or more compounds present in the solution. - View Dependent Claims (34, 35, 36, 37, 38, 39)
- An apparatus for measuring the concentration of a substance in a solution, said substance having an absorption at 300 nm or less, comprising;
Specification