Method for Overproducing Specific Recombinant Protein with P. Cinnabarinus Monokaryotic Strains
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Abstract
The use of monokaryotic strains of filamentous fungi of the Pycnoporus species of the basidiomycete group, for implementing a method for preparing a specific recombinant protein, the method being carried out by overexpressing the gene encoding the protein in the Pycnoporus monokaryotic strain.
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Citations
39 Claims
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1-20. -20. (canceled)
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21. Method for preparing a specific recombinant protein, said method being carried out by overexpression of the gene encoding for this specific protein in a monokaryotic strain of filamentous fungi of the species Pycnoporus of the basidiomycete group, and comprises:
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a stage of culturing the abovementioned monokaryotic strain of Pycnoporus, said strain being transformed using an expression vector containing the gene encoding for the specific recombinant protein, the expression of which is placed under the control of a promoter corresponding to an endogenous promoter of the abovementioned fungi, or of a different promoter (also designated exogenous promoter), said promoter being constitutive or inducible, if appropriate a stage of induction of the abovementioned promoter, when the latter is inducible, the recovery, and, if appropriate, the purification of the specific recombinant protein, produced in the culture medium. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. Nucleotide sequence encoding for the pLac promoter of the endogenous laccase of Pycnoporus cinnabarinus, and corresponding to the sequence SEQ ID NO:
- 3, or any sequence derived from this promoter by substitution, addition or suppression of one or more nucleotides and retaining the property of being a promoter of the expression of sequences.
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35. Expression vector characterized in that it comprises the sequence SEQ ID NO:
- 3 encoding for the pLac promoter of the endogenous laccase of Pycnoporus cinnabarinus.
- View Dependent Claims (36, 37, 38, 39)
Specification