Shape memory thermoplastics and polymer networks for tissue engineering

• US 20080305545A1
• Filed: 04/10/2008
• Published: 12/11/2008
• Est. Priority Date: 05/31/2000
• Status: Abandoned Application

• Current Assignee: Helmholtz-Zentrum Geesthacht Zentrum Fur Material-Und Kustenforschung GmbH
• Original Assignee: mNemoscience GmbH
• Inventors: Lendlein, Andreas, Kratz, Karl, Knischka, Ralf
• Application Number:
  US12/081,104
• Publication Number:
  US 20080305545A1
• Time in Patent Office: Days
• US Class Current: 435/377
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: B29C 61/06 Making preforms having internal stresses, e.g. plastic memory
• CPC Class Codes: B29K 2995/006 Bio-degradable, e.g. bioabsorbable, bioresorbable or bioerodible
• CPC Class Codes: C08L 101/00 Compositions of unspecified macromolecular compounds
• CPC Class Codes: C08L 2201/12 Shape memory
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence

Abstract

Methods and compositions are described herein for reconstruction of different functional tissues. Dissociated cells, differentiated cells, adult mesenchymal stem cells or embryonic stem cells are seeded on a scaffold. The scaffold will consist of a biocompatible, biodegradable shape memory (“SM”) polymers. In addition bioactive substances may be incorporated in the scaffold. Thermoplastic as well as thermoset materials with SM-effect can be used. The shape memory effect will be applied as an interactive link between the cells and the used polymeric scaffold. The degradation kinetics as well as shape memory transition temperature will be tailored by adjusting to monomer ratios of the co-oligomers. The shape memory effect will be used to create a degradation or release of bioactive substances on demand, induce forces on seeded cells or induce proliferation and differentiation of cells.

Claims

1-27. -27. (canceled)

28. A method of inducing the release of at least one bioactive material on demand comprising inducing a shape memory change in a cell seeded, shape memory polymer containing implant with a stimulus, wherein said inducing comprises exposing said implant to at least one stimulus.

29. The method of claim 28, wherein said cell seeded, shape memory polymer containing implant is seeded by proliferating cells on a shape memory polymer containing implant

30. The method of claim 28, wherein said release of said at least bioactive material alters at least one of cell attachment, cell proliferation, cell structure, cell differentiation and combinations thereof.

31. The method of claim 28, wherein said shape memory polymer is biodegradable.

32. The method according to claim 28, wherein said cells are dissociated cells.

33. The method according to claim 28, wherein said shape memory polymer is free of lactide units.

34. The method according to claim 28, wherein the rate of release of said at least one bioactive material is altered by inducing said shape memory change.

35. The method of claim 28, wherein said shape memory polymer is in the form of particles having a size from approximately 10 nm to approximately 2000 microns.

36. The method of claim 28, wherein said implant is in the form of at least one of a sphere, a pellet, a rod, a film and a tube.

37. The method of claim 28, wherein said at least one stimulus is heat.