Methods of determining hair follicle inductive properties

• US 20080311044A1
• Filed: 06/12/2007
• Published: 12/18/2008
• Est. Priority Date: 06/12/2007
• Status: Active Grant

• Current Assignee: Aderans Research Institute Incorporated
• Original Assignee: Aderans Research Institute Incorporated
• Inventors: Parimoo, Satish, Stenn, Kurt Stricker, Zheng, Ying
• Granted Patent: US 7,985,537 B2
• Time in Patent Office: Days
• US Class Current: 424/9.2
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 2267/03 Animal model, e.g. for test or diseases
• CPC Class Codes: A01K 67/0271 Chimeric vertebrates, e.g. comprising exogenous cells
• CPC Class Codes: A61K 49/0006 Skin tests, e.g. intradermal testing, test strips, delayed hypersensitivity
• CPC Class Codes: G01N 33/5082 Supracellular entities, e.g. tissue, organisms

Abstract

The present invention provides a method for determining hair inductive properties of a composition comprising injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal and determining whether at least one hair follicle forms.

Claims

1. A method for determining hair inductive properties of a composition comprising:

injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells are from a first species and epidermal cells are from a second species; and

wherein the first species is different from the second species; and

determining whether at least one hair follicle forms, wherein the hair follicle is associated with at least one sebaceous gland; and

wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.

2. The method of claim 1, wherein the mammal is an immunocompromised mouse.

3. The method of claim 1, wherein the first species is mouse and the second species is human.

4. The method of claim 1, wherein the first species is human and the second species is mouse.

5. The method of claim 1, wherein from about one million to about five million cells are injected.

6. The method of claim 1, wherein the composition further comprises a carrier.

7. The method of claim 1, wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.

8. The method of claim 1, wherein the composition is injected into the deep dermis of the mammal.

9. The method of claim 1, wherein the composition is injected into a confined space.

10. A method for determining hair inductive properties of a composition comprising:

injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells are from a first species and epidermal cells are from a second species and wherein the first species is different from the second species; and

determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla; and

wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.

11. The method of claim 10, wherein the mammal is an immunocompromised mouse.

12. The method of claim 10, wherein the first species is mouse and the second species is human.

13. The method of claim 10, wherein the first species is human and the second species is mouse.

14. The method of claim 10, wherein from about one million to about five million cells are injected.

15. The method of claim 10, wherein the composition further comprises a carrier.

16. The method of claim 10, wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.

17. The method of claim 10, wherein the composition is injected into the deep dermis of the mammal.

18. The method of claim 10, wherein the composition is injected into a confined space.

19. A method for determining hair inductive properties of a composition comprising:

injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells and epidermal cells are from human; and

determining whether at least one hair follicle forms, wherein the hair follicle is associated with at least one sebaceous gland; and

wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.

20. The method of claim 19, wherein the mammal is an immunocompromised mouse.

21. The method of claim 19, wherein from about one to about five million cells are injected.

22. The method of claim 19, wherein the composition further comprises a carrier.

23. The method of claim 19, wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.

24. The method of claim 19, wherein the composition is injected into the deep dermis of the mammal.

25. The method of claim 19, wherein the composition is injected into a confined space.

26. A method for determining hair inductive properties of a composition comprising:

injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells and epidermal cells are human; and

determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla; and

wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.

27. The method of claim 26, wherein the mammal is an immunocompromised mouse.

28. The method of claim 26, wherein from about one to about five million cells are injected.

29. The method of claim 26, wherein the composition further comprises a carrier.

30. The method of claim 26, wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.

31. The method of claim 26, wherein the composition is injected into the deep dermis of the mammal.

32. The method of claim 26, wherein the composition is injected into a confined space.